Celeste Donato1, Le Thi Hoi2, Nguyen Thi Hoa3, Tran Mai Hoa2, Le Van Duyet2, Ta Thi Dieu Ngan2, Nguyen Van Kinh2, Nguyen Vu Trung2, Dhanasekaran Vijaykrishna4. 1. Duke-NUS Medical School, 8 College Rd, Singapore. 2. National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam. 3. Department of Clinical Microbiology, Hanoi Medical University, Vietnam. 4. Duke-NUS Medical School, 8 College Rd, Singapore; Department of Pathology, Singapore General Hospital, Singapore. Electronic address: vijay.dhanasekaran@duke-nus.edu.sg.
Abstract
BACKGROUND: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities. METHODS: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected. RESULTS: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases. CONCLUSIONS: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.
BACKGROUND:Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities. METHODS: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected. RESULTS: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases. CONCLUSIONS: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.
Authors: Sacha Stelzer-Braid; Matthew Wynn; Richard Chatoor; Matthew Scotch; Vidiya Ramachandran; Hooi-Ling Teoh; Michelle A Farrar; Hugo Sampaio; Peter Ian Andrews; Maria E Craig; C Raina MacIntyre; Hemalatha Varadhan; Alison Kesson; Philip N Britton; James Newcombe; William D Rawlinson Journal: J Clin Virol Date: 2019-11-17 Impact factor: 3.168
Authors: Nguyen Thi Thanh Thao; Celeste Donato; Vu Thi Huyen Trang; Nguyen Trung Kien; Ph M Mai Thuy Trang; Tran Quoc Khanh; Dang Thi Nguyet; October M Sessions; Hoang Quoc Cuong; Phan Trong Lan; Vu Thi Que Huong; H Rogier van Doorn; Dhanasekaran Vijaykrishna Journal: J Infect Dis Date: 2017-12-12 Impact factor: 5.226
Authors: Rubén González-Sanz; Didac Casas-Alba; Cristian Launes; Carmen Muñoz-Almagro; María Montserrat Ruiz-García; Mercedes Alonso; María José González-Abad; Gregoria Megías; Nuria Rabella; Margarita Del Cuerpo; Mónica Gozalo-Margüello; Alejandro González-Praetorius; Ana Martínez-Sapiña; María José Goyanes-Galán; María Pilar Romero; Cristina Calvo; Andrés Antón; Manuel Imaz; Maitane Aranzamendi; Águeda Hernández-Rodríguez; Antonio Moreno-Docón; Sonia Rey-Cao; Ana Navascués; Almudena Otero; María Cabrerizo Journal: Euro Surveill Date: 2019-02
Authors: Son T Chu; Kyousuke Kobayashi; Xiuqiong Bi; Azumi Ishizaki; Tu T Tran; Thuy T B Phung; Chung T T Pham; Lam V Nguyen; Tuan A Ta; Dung T K Khu; Masanobu Agoh; An N Pham; Satoshi Koike; Hiroshi Ichimura Journal: Sci Rep Date: 2020-01-13 Impact factor: 4.379