| Literature DB >> 27147463 |
Joana F S Pereira1,2, Nikhil T Awatade2, Cláudia A Loureiro1,2, Paulo Matos1,2, Margarida D Amaral2, Peter Jordan3,4.
Abstract
Cellular models are important tools in various research areas related to colorectal biology and associated diseases. Herein, we review the most widely used cell lines and the different techniques to grow them, either as cell monolayer, polarized two-dimensional epithelia on membrane filters, or as three-dimensional spheres in scaffold-free or matrix-supported culture conditions. Moreover, recent developments, such as gut-on-chip devices or the ex vivo growth of biopsy-derived organoids, are also discussed. We provide an overview on the potential applications but also on the limitations for each of these techniques, while evaluating their contribution to provide more reliable cellular models for research, diagnostic testing, or pharmacological validation related to colon physiology and pathophysiology.Entities:
Keywords: 3D culture; Cell culture model; Cell polarization; Cell spheroids; Colon; Colorectal cancer; Cystic fibrosis; Epithelial chloride transport; Inflammatory bowel disease; Organoids; Small intestine
Mesh：
Year: 2016 PMID： 27147463 DOI： 10.1007/s00018-016-2258-2
Source DB: PubMed Journal: Cell Mol Life Sci ISSN： 1420-682X Impact factor: 9.261