| Literature DB >> 27147035 |
Wei Xie1, Henning F Horn2, Graham D Wright3.
Abstract
Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.Entities:
Keywords: Linker of the nucleoskeleton and cytoskeleton (LINC) complex; Nuclear envelope (NE); Nuclear lamins; Nuclear pore complex (NPC); Photoactivated localization microscopy (PALM); Single-molecule localization microscopy (SMLM); Stochastic optical reconstruction microscopy (STORM); Structured illumination microscopy (SIM); Superresolution microscopy (SRM); Synaptonemal complex
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Year: 2016 PMID： 27147035 DOI： 10.1007/978-1-4939-3530-7_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745