| Literature DB >> 27145783 |
Guilherme Vargas Bochi1,2,3, Vanessa Dorneles Torbitz4, Roberto Christ Vianna Santos5, Monica Cubillos-Rojas6, José Luis Rosa López6, Anna Maria Siebel7, Patrícia Gomes8, Jarbas Rodrigues de Oliveira9, Rafael Noal Moresco4,10.
Abstract
Fenton reaction is a new mechanism able to generate advanced oxidation protein products (AOPPs) by exposing the human serum albumin to the Fenton system. Here, we characterized the effects of Fenton reaction-generated advanced oxidation protein products (AOPP-FR) on the gene transcription of the nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), and interleukin-6 (IL-6) in human embryonic kidney cells (HEK 293). To investigate the effects of AOPP-FR and AOPP-HOCl on transcription of inflammatory genes, the NF-κB, COX-2, and IL-6 luciferase promoter activities were analyzed. AOPP-FR and AOPP-HOCl were able to induce the activation of the gene transcription of NF-κB, COX-2, and IL-6 in HEK 293 cells. However, the effects of AOPP-FR were significantly higher than the effects of AOPP-HOCl in relation to COX-2 and IL-6. AOPP-FR induces the activation of the gene transcription of NF-κB, COX-2, and IL-6 and may represent a novel pathogenic mediator of inflammation in kidney.Entities:
Keywords: HEK 293; advanced oxidation protein products; fenton reaction; hypochlorous acid inflammation
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Year: 2016 PMID： 27145783 DOI： 10.1007/s10753-016-0360-0
Source DB: PubMed Journal: Inflammation ISSN： 0360-3997 Impact factor: 4.092