| Literature DB >> 27145125 |
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Abstract
[This corrects the article DOI: 10.1371/journal.pone.0149995.].Entities:
Year: 2016 PMID: 27145125 PMCID: PMC4856372 DOI: 10.1371/journal.pone.0155281
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
List of proteins that selectively interact with unphosphorylatable form of rpS6.
Whole cell extract from HEK293 cells infected with pS6[5S]-GFP, pS6[5A]-GFP, pS6[5D]-GFP or pEGFP-N1, was subjected to GFP pull-down, and the bound proteins were size fractionated by SDS-polyacrlamide gel electrophoresis. Mass spectrometric analysis of proteins in each lane was performed as described in “material and Methods” and proteins, selectively bound to pS6[5A]-GFP in two independent experiments, are presented (numbers separated by slash [/] represent results obtained in each of the two individual analyses).
| Gene name | Protein | Function | Location | Area | Coverage | No. of unique peptides |
|---|---|---|---|---|---|---|
| PC4 and SFRS1-interacting protein 1 (LEDGF) | Repair of DNA double-strand breaks | Nucleus | 1.141E6/1.705E7 | 5.28/17.55 | 3/8 | |
| Topoisomerase (DNA) II beta | DNA replication, transcription and repair | Nucleus | 7.922E6/2.783E7 | 19.62/8.98 | 18/7 | |
| Serine/arginine-rich splicing factor 4 | Splicing | Nucleus | 2.327E6/3.816E7 | 7.89/11.54 | 3/2 | |
| ATP-binding cassette, sub-family D (ALD), member 3 | Transporter (?) | Peroxysomal and mitochondrial membranes | 1.584E6/9.683E6 | 6.83/3.95 | 3/2 | |
| NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 9 | Accessory subunit of Complex I | Mitochondria | 5.180E5/8.213E6 | 8.49/9.28 | 3/3 | |
| Glutaminyl-tRNA synthetase | Translation | Cytoplasm | 1.596E6/5.299E6 | 6.32/5.42 | 4/3 |
a Area—displays the average area of the three unique peptides with the largest peak area, based on extracted ion currents (XICs).
b Coverage—displays the percentage of the protein sequence covered by identified peptides.
c No. of unique peptides—Displays the number of peptide sequences unique to a protein group.