Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry.

Literature DB >> 27145073

Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry.

Abstract

Signal transduction across cell membranes often requires assembly of heteromeric receptors with defined stoichiometry. In this issue of Structure, Morales-Perez et al. (2016) present elegant methods for the expression of heteromeric nicotinic acetylcholine receptors with a defined α4β2 stoichiometry involving controlled baculovirus-mediated transduction and subunit counting by measurement of two fluorescent signals.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：
Receptors, Nicotinic

Year: 2016 PMID： 27145073 PMCID： PMC5266497 DOI： 10.1016/j.str.2016.04.004

Source DB: PubMed Journal: Structure ISSN： 0969-2126 Impact factor: 5.006

Keyword Cloud
References

9 in total

1. Refined structure of the nicotinic acetylcholine receptor at 4A resolution.

Authors: Nigel Unwin
Journal: J Mol Biol Date: 2005-01-25 Impact factor: 5.469

2. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins.

Authors: Toshimitsu Kawate; Eric Gouaux
Journal: Structure Date: 2006-04 Impact factor: 5.006

3. Pentameric concatenated (alpha4)(2)(beta2)(3) and (alpha4)(3)(beta2)(2) nicotinic acetylcholine receptors: subunit arrangement determines functional expression.

Authors: A-L Carbone; M Moroni; P-J Groot-Kormelink; I Bermudez
Journal: Br J Pharmacol Date: 2009-03 Impact factor: 8.739

Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry.

1. Refined structure of the nicotinic acetylcholine receptor at 4A resolution.

2. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins.

3. Pentameric concatenated (alpha4)(2)(beta2)(3) and (alpha4)(3)(beta2)(2) nicotinic acetylcholine receptors: subunit arrangement determines functional expression.

4. Crystal structure of a heterotetrameric NMDA receptor ion channel.

5. Structure and gating mechanism of the acetylcholine receptor pore.

6. Alternate stoichiometries of alpha4beta2 nicotinic acetylcholine receptors.

7. Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins.

8. Structure and organization of heteromeric AMPA-type glutamate receptors.

9. NMDA receptor structures reveal subunit arrangement and pore architecture.