Yasemin Yuksel1, Mustafa Guven2, Burak Kaymaz3, Muserref Hilal Sehitoglu4, Adem Bozkurt Aras2, Tarik Akman2, Murat Tosun5, Murat Cosar2. 1. a In Vitro Fertilization Unit, ZekaiTahirBurak Women's Health Education and Research Hospital , Ankara , Turkey. 2. b Faculty of Medicine, Department of Neurosurgery , Canakkale Onsekiz Mart University , Canakkale , Turkey. 3. c Faculty of Medicine, Department of Orthopaedic , Canakkale Onsekiz Mart University , Canakkale , Turkey. 4. d Faculty of Medicine, Department of Medical Biochemistry , Canakkale Onsekiz Mart University , Canakkale , Turkey. 5. e Faculty of Medicine, Department of Histology & Embryology , AfyonKocatepe University , Afyon , Turkey.
Abstract
AIM: The purpose of this study was to evaluate the possible protective/therapeutic effects of aloe vera (AV) on ischemia-reperfusion injury (I/R) of spinal cord in rats. MATERIALS AND METHODS: A total of 28 Wistar Albino rats were divided into four random groups of equal number (n = 7). Group I (control) had no medication or surgery; Group II underwent spinal cord ischemia and was given no medication; Group III was administered AV by gastric gavage for 30 days as pre-treatment; Group IV was administered single dose intraperitoneal methylprednisolone (MP) after the ischemia. Nuclear respiratory factor-1 (NRF1), malondialdehyde (MDA) and superoxide dismutase (SOD) levels were evaluated. Tissue samples were examined histopathologically and neuronal nitric oxide synthase (nNOS) and nuclear factor-kappa B (NF-κB) protein expressions were assessed by immunohistochemical staining. RESULTS: NRF1 and SOD levels of ischemia group were found to be lower compared to the other groups. MDA levels significantly increased after I/R. Treatment with AV and MP resulted in reduced MDA levels and also alleviated hemorrhage, edema, inflammatory cell migration and neurons were partially protected from ischemic injury. When AV treatment was compared with MP, there was no statistical difference between them in terms of reduction of neuronal damage. I/R injury increased NF-κB and nNOS expressions. AV and MP treatments decreased NF-κB and nNOS expressions. CONCLUSIONS: It was observed that aloe vera attenuated neuronal damage histopathologically and biochemically as pretreatment. Further studies may provide more evidence to determine the additional role of aloe vera in spinal cord ischemia reperfusion injury.
AIM: The purpose of this study was to evaluate the possible protective/therapeutic effects of aloe vera (AV) on ischemia-reperfusion injury (I/R) of spinal cord in rats. MATERIALS AND METHODS: A total of 28 Wistar Albino rats were divided into four random groups of equal number (n = 7). Group I (control) had no medication or surgery; Group II underwent spinal cord ischemia and was given no medication; Group III was administered AV by gastric gavage for 30 days as pre-treatment; Group IV was administered single dose intraperitoneal methylprednisolone (MP) after the ischemia. Nuclear respiratory factor-1 (NRF1), malondialdehyde (MDA) and superoxide dismutase (SOD) levels were evaluated. Tissue samples were examined histopathologically and neuronal nitric oxide synthase (nNOS) and nuclear factor-kappa B (NF-κB) protein expressions were assessed by immunohistochemical staining. RESULTS: NRF1 and SOD levels of ischemia group were found to be lower compared to the other groups. MDA levels significantly increased after I/R. Treatment with AV and MP resulted in reduced MDA levels and also alleviated hemorrhage, edema, inflammatory cell migration and neurons were partially protected from ischemic injury. When AV treatment was compared with MP, there was no statistical difference between them in terms of reduction of neuronal damage. I/R injury increased NF-κB and nNOS expressions. AV and MP treatments decreased NF-κB and nNOS expressions. CONCLUSIONS: It was observed that aloe vera attenuated neuronal damage histopathologically and biochemically as pretreatment. Further studies may provide more evidence to determine the additional role of aloe vera in spinal cord ischemia reperfusion injury.
Authors: Bora Tastekin; Aykut Pelit; Sait Polat; Abdullah Tuli; Leman Sencar; Mustafa Muhlis Alparslan; Yusuf Kenan Daglioglu Journal: Evid Based Complement Alternat Med Date: 2018-05-16 Impact factor: 2.629
Authors: Gholam Hossein Farjah; Masoumeh Mohammad Pour; Mohammad Hassan Khadem-Ansari; Mojtaba Karimipour; Bagher Pourheidar Journal: Vet Res Forum Date: 2018-06-15 Impact factor: 1.054