Malathi Narayan1, Kent W Seeley2, Umesh K Jinwal1. 1. Department of Pharmaceutical Sciences, College of Pharmacy, Byrd Alzheimer's Institute, University of South Florida-Health, Tampa, FL 33613, USA. 2. Florida Center of Excellence for Drug Discovery & Innovation at the University of South Florida, 3720 Spectrum Boulevard, Suite 303, IDR Building, Tampa, FL 33612, USA.
Abstract
AIM: Amyotrophic lateral sclerosis (ALS) is a debilitating fatal neurodegenerative disorder. 90-95% of ALS cases are sporadic with no clear risk factors associated with the disease. Identification of biomarkers associated with ALS may lead to early detection and make it more amenable to therapeutic intervention. MATERIALS & METHODS: SILAC was used to quantitatively analyze the proteomes of ALS and control human fibroblasts. RESULTS: Out of total of 861 proteins identified, 33 were found to be differentially regulated. ApoB48 and Hsp20 were downregulated, while Fibulin-1 was upregulated. CONCLUSION: We report the differential regulation of these proteins in ALS fibroblasts, and their potential as novel biomarkers and possible drug targets for ALS.
AIM: Amyotrophic lateral sclerosis (ALS) is a debilitating fatal neurodegenerative disorder. 90-95% of ALS cases are sporadic with no clear risk factors associated with the disease. Identification of biomarkers associated with ALS may lead to early detection and make it more amenable to therapeutic intervention. MATERIALS & METHODS: SILAC was used to quantitatively analyze the proteomes of ALS and control human fibroblasts. RESULTS: Out of total of 861 proteins identified, 33 were found to be differentially regulated. ApoB48 and Hsp20 were downregulated, while Fibulin-1 was upregulated. CONCLUSION: We report the differential regulation of these proteins in ALS fibroblasts, and their potential as novel biomarkers and possible drug targets for ALS.
Entities:
Keywords:
Apo B48; SILAC; amyotrophic lateral sclerosis (ALS); biomarker; mass spectrometry; proteomics
Authors: Samuel A Danziger; Leslie R Miller; Karanbir Singh; G Adam Whitney; Elaine R Peskind; Ge Li; Robert J Lipshutz; John D Aitchison; Jennifer J Smith Journal: PLoS One Date: 2017-06-08 Impact factor: 3.240
Authors: Qiuying Chen; Csaba Konrad; Davinder Sandhu; Dipa Roychoudhury; Benjamin I Schwartz; Roger R Cheng; Kirsten Bredvik; Hibiki Kawamata; Elizabeth L Calder; Lorenz Studer; Steven M Fischer; Giovanni Manfredi; Steven S Gross Journal: Neurobiol Dis Date: 2020-08-01 Impact factor: 5.996