Zarmina Muhammad1, Abida Raza2, Sana Ghafoor3, Ayesha Naeem4, Syeda Sohaila Naz5, Sundus Riaz6, Wajiha Ahmed7, Nosheen Fatima Rana8. 1. School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, H-12 Campus, Islamabad, Pakistan. Electronic address: zarmina.muhammad@gmail.com. 2. Nuclear Medicine, Oncology and Radiotherapy Institute, Islamabad, Pakistan. Electronic address: abida_rao@yahoo.com. 3. School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, H-12 Campus, Islamabad, Pakistan. Electronic address: ghafoorsana@googlemail.com. 4. School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, H-12 Campus, Islamabad, Pakistan. Electronic address: ayesha.n.mehmood@gmail.com. 5. Nuclear Medicine, Oncology and Radiotherapy Institute, Islamabad, Pakistan. Electronic address: organicsoul84@gmail.com. 6. School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, H-12 Campus, Islamabad, Pakistan. Electronic address: sundusriaz_fuuast@yahoo.com. 7. School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, H-12 Campus, Islamabad, Pakistan. Electronic address: 13msbmswahmad@smme.edu.pk. 8. School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, H-12 Campus, Islamabad, Pakistan. Electronic address: nosheen.fatima@smme.nust.edu.pk.
Abstract
BACKGROUND: Nanocarriers endow tremendous benefits to the drug delivery systems depending upon the specific properties of either component. These benefits include, increase in the drug blood retention time, reduced efflux, additional toxicity and targeted delivery. Methotrexate (MTX) is clinically used for cancer treatment. Higher dosage of MTX results in hepatic and renal toxicity. In this study methotrexate silver nanoparticles (Ag-MTX) coated with polyethylene glycol (PEG) are synthesized and characterized. Their anticancer activity and biocompatibility is also evaluated. RESULTS: Ag-MTX nanoparticles are synthesized by chemical reduction method. They are characterized by Ultraviolet-Visible Spectroscopy and Fourier Transform Infrared Spectroscopy. Average size of PEG coated Ag-MTX nanoparticles (PEG-Ag-MTX nanoparticles) is 12nm. These particles exhibited improved anticancer activity against MCF-7 cell line. Hemolytic activity of these particles was significantly less than MTX. CONCLUSION: PEG-Ag-MTX nanoparticles are potential nanocarrier of methotrexate which may offer MTX based cancer treatment with reduced side effects. In-vivo investigations should be carried out to explore them in detail.
BACKGROUND: Nanocarriers endow tremendous benefits to the drug delivery systems depending upon the specific properties of either component. These benefits include, increase in the drug blood retention time, reduced efflux, additional toxicity and targeted delivery. Methotrexate (MTX) is clinically used for cancer treatment. Higher dosage of MTX results in hepatic and renal toxicity. In this study methotrexate silver nanoparticles (Ag-MTX) coated with polyethylene glycol (PEG) are synthesized and characterized. Their anticancer activity and biocompatibility is also evaluated. RESULTS:Ag-MTX nanoparticles are synthesized by chemical reduction method. They are characterized by Ultraviolet-Visible Spectroscopy and Fourier Transform Infrared Spectroscopy. Average size of PEG coated Ag-MTX nanoparticles (PEG-Ag-MTX nanoparticles) is 12nm. These particles exhibited improved anticancer activity against MCF-7 cell line. Hemolytic activity of these particles was significantly less than MTX. CONCLUSION:PEG-Ag-MTX nanoparticles are potential nanocarrier of methotrexate which may offer MTX based cancer treatment with reduced side effects. In-vivo investigations should be carried out to explore them in detail.
Authors: Ahmed A H Abdellatif; Mansour Alsharidah; Osamah Al Rugaie; Hesham M Tawfeek; Nahla Sameh Tolba Journal: Drug Des Devel Ther Date: 2021-05-13 Impact factor: 4.162