| Literature DB >> 27130837 |
Shiquan Gan1,2, Zhaoqin Huang1,2, Ning Liu1,3, Renxiang Su1,2, Guie Xie1,2, Beibei Zhong1,2, Kai Zhang1,2, Shang Wang1,2, Xiang Hu1,2, Jian Zhang1,2, Shuanglin Xiang1,2.
Abstract
MicroRNA-140-5p (miRNA-140-5p) is important for embryonic bone development. In this study, we found that miRNA-140-5p and its binding site in the 3'UTR of bone morphogenetic protein 2 (BMP-2) are highly conserved among vertebrates, and miRNA-140-5p negatively regulates both zebrafish and human BMP-2 genes. Microinjection of miRNA-140-5p or BMP-2b morpholino into zebrafish embryos led to a similar phenotype, including shortened tails, curved trunks, and defects in cranial cartilage. Moreover, miRNA-140-5p injection induced zebrafish embryo malformation that could be significantly rescued by microinjection of BMP-2 mRNA. In conclusion, our results indicated that miRNA-140-5p regulates zebrafish embryonic bone development via targeting BMP-2.Entities:
Keywords: BMP-2; bone and cartilage development; embryos; microRNA-140-5p; zebrafish
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Year: 2016 PMID： 27130837 DOI： 10.1002/1873-3468.12190
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124