Aldo Amaro-Reyes1, Jorge Gracida1, Nelson Huizache-Peña2, Norberto Elizondo-García3, José Salazar-Martínez3, Blanca E García Almendárez1, Carlos Regalado4. 1. Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas s/n Col. Las Campanas, 76010 Querétaro, Querétaro, Mexico. 2. Universidad Politécnica de Pachuca, Carretera Pachuca-Ciudad Sahagún Km. 20, Ex-Hacienda de Santa Bárbara, 43830 Zempoala, Hidalgo, Mexico. 3. Forrajera Elizondo, S.A. de C.V., Carlos Salinas de Gortari 600, Apodaca Centro, 66600 Apodaca, Nuevo León, Mexico. 4. Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas s/n Col. Las Campanas, 76010 Querétaro, Querétaro, Mexico. Electronic address: regcarlos@gmail.com.
Abstract
Solid state fermentation (SSF) is used to produce industrial enzymes. The objective of this study was to use a co-culture of Aspergillus niger GS1 and Trichoderma reesei, grown on a mixture of Bermuda grass and corn cob to obtain fermented forage (FF) rich in hydrolytic enzymes, as a value added ingredient for animal feed. FPase, amylase and xylanase productivities (dry matter, DM) were 8.8, 181.4, and 42.1Ug(-1)h(-1), respectively (1U=reducing sugars released min(-1)), after 12-16h of SSF with C/N=60. Cellulose, hemicellulose and lignin decreased 1.6-, 2.7- and 1.9-fold (DM), respectively. In vitro ruminal and true digestibility of DM was improved 2.4- and 1.4-fold. Ruminal digestion of FF reduced 1.32-fold the acetate:propionate ratio, which may reduce the environmental impact of ruminants feeding. On-site hydrolytic enzymes productivity using SSF without enzymes extraction could be of economic potential for digestibility improvement in animal feed.
Solid state fermentation (SSF) is used to produce industrial enzymes. The objective of this study was to use a co-culture of n class="Species">Aspergillus niger GS1 and Trichoderma reesei, grown on a mixture of Bermuda grass and corn cob to obtain fermented forage (FF) rich in hydrolytic enzymes, as a value added ingredient for animal feed. FPase, amylase and xylanase productivities (dry matter, DM) were 8.8, 181.4, and 42.1Ug(-1)h(-1), respectively (1U=reducing sugars released min(-1)), after 12-16h of SSF with C/N=60. Cellulose, hemicellulose and lignin decreased 1.6-, 2.7- and 1.9-fold (DM), respectively. In vitro ruminal and true digestibility of DM was improved 2.4- and 1.4-fold. Ruminal digestion of FF reduced 1.32-fold the acetate:propionate ratio, which may reduce the environmental impact of ruminants feeding. On-site hydrolytic enzymes productivity using SSF without enzymes extraction could be of economic potential for digestibility improvement in animal feed.
Authors: Martha María Arevalos-Sánchez; Adrián Omar Maynez-Perez; Felipe A Rodríguez-Almeida; José Alfredo Martínez-Quintana; Fidel Alejandro Sanchez-Flores; Monserrath Felix-Portillo; América Chavéz-Martínez; Myrna Elena Olvera-García; Oscar Ruiz-Barrera; Agustín Corral-Luna Journal: BMC Biotechnol Date: 2021-03-23 Impact factor: 2.563