Ahmed Abdelsalam1, Laila Rashed2, Tarek Salman3, Lamiaa Hammad4, Dina Sabry2. 1. Department of Biochemistry, Faculty of Pharmacy, Misr International University, Cairo, Egypt. ahmed.gouda@miuegypt.edu.eg. 2. Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt. 3. Department of Biochemistry, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt. 4. Department of Biochemistry, Faculty of Pharmacy, Misr International University, Cairo, Egypt.
Abstract
OBJECTIVE: The aim of this study was to find an association between serum concentration of vitamin D and vitamin D receptor (VDR) polymorphisms to achieve a sustained virological response (SVR). METHODS: We conducted a case-control study in which 250 participants were recruited and divided into three groups (100 chronic hepatitis C [CHC] patients who achieved SVR, 100 CHC patients who did not achieve SVR and 50 apparently healthy individuals as controls). Blood samples were collected to measure serum vitamin D concentration, and four VDR polymorphisms (FokI, ApaI, TaqI, and BsmI) were detected using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: Non-responders were found to have significantly low vitamin D concentration compared with responders and control groups. Concerning VDR polymorphisms, both FokI and TaqI polymorphisms were associated with successful treatment. CONCLUSION: Vitamin D concentration, FokI, and TaqI may be considered as the predictors for the response of CHC patients to a combination therapy of pegylated interferon and ribavirin.
OBJECTIVE: The aim of this study was to find an association between serum concentration of vitamin D and vitamin D receptor (VDR) polymorphisms to achieve a sustained virological response (SVR). METHODS: We conducted a case-control study in which 250 participants were recruited and divided into three groups (100 chronic hepatitis C [CHC] patients who achieved SVR, 100 CHCpatients who did not achieve SVR and 50 apparently healthy individuals as controls). Blood samples were collected to measure serum vitamin D concentration, and four VDR polymorphisms (FokI, ApaI, TaqI, and BsmI) were detected using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: Non-responders were found to have significantly low vitamin D concentration compared with responders and control groups. Concerning VDR polymorphisms, both FokI and TaqI polymorphisms were associated with successful treatment. CONCLUSION:Vitamin D concentration, FokI, and TaqI may be considered as the predictors for the response of CHCpatients to a combination therapy of pegylated interferon and ribavirin.
Authors: Sunil Gupta; Scott A Read; Nicholas A Shackel; Lionel Hebbard; Jacob George; Golo Ahlenstiel Journal: Cells Date: 2019-06-17 Impact factor: 6.600
Authors: Nghiem Xuan Hoan; Nguyen Khuyen; Dao Phuong Giang; Mai Thanh Binh; Nguyen Linh Toan; Do Tuan Anh; Ngo Tat Trung; Mai Hong Bang; Christian G Meyer; Thirumalaisamy P Velavan; Le Huu Song Journal: BMC Med Genet Date: 2019-12-21 Impact factor: 2.103