Alterations of collagen-producing cells in human pituitary adenomas.

Extracellular matrix (ECM) is essential in tissue physiology and pathologic conditions such as tumorigenesis. ECM affects tumor cell behavior, proliferation, and metastasis. Pituitary adenomas vary in their clinical characteristics, including ECM deposition. However, the mechanism of desmoplasia in pituitary adenoma is not well understood. The present study focused on the principal component of ECM, collagen, and attempted to characterize collagen-producing cells in pituitary adenomas. Specimens of human pituitary adenomas and control pituitary were obtained during surgery. In situ hybridization for collagen I and III and immunohistochemistry for α-smooth muscle actin (a pericyte marker) and cytokeratin (an epithelial cell marker) were performed. The results showed that pericytes were the sole collagen-producing cells in control pituitary, while four types of collagen-producing cells were present in pituitary adenomas: pericytes, myofibroblasts, fibroblasts, and newly characterized "myoepithelial-like cells". Azan staining showed that fibrous matrix deposition varied among pituitary adenomas and that the area of fibrosis was associated with the number and types of collagen-producing cells. These results suggest that changes in the number and type of collagen-producing cells influence ECM arrangement, which may in turn reflect pathologic characteristics in pituitary adenomas.