BACKGROUND: Inflammation may contribute to cardiovascular disease (CVD) among antiretrovirally suppressed HIV-infected individuals. We assessed relationships of monocyte, CD8 T-cell activation and plasma biomarkers to changes in carotid artery intima-media thickness (CIMT). METHODS: Longitudinal study of HIV-infected subjects ≥40 years and on stable antiretroviral therapy (ART) ≥3 months. Peripheral blood mononuclear cells were immunophenotyped by multiparameteric flow cytometry to quantify classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), non-classical (CD14(low/+)CD16(++)) and transitional (CD14(+)CD16(-)) monocyte subsets and activated (CD38(+)HLA-DR(+)) CD8(+) T-cells at baseline. Plasma biomarkers were assessed by multiplex Luminex assay. High-resolution B-mode ultrasounds of right carotid arteries were obtained. Changes in CIMT over two years at the right common carotid artery (CIMTCCA) and right bifurcation (CIMTBIF) were outcome variables. RESULTS: We studied 50 subjects: 84% male, median age 49 (Q1, Q3; 46, 56) years, median CD4 count 461 (317, 578) cells/mm(3), and with HIV RNA ≤ 50 copies/mL in 84%. Change in CIMTBIF correlated with log values of baseline absolute count of non-classical monocytes (r = 0.37, p = 0.020), and with MCP-1 (r = 0.42, p = 0.0024) and TNF-α (r = 0.30, p = 0.036) levels. In multivariable linear regression, only non-classical monocytes and MCP-1 predicted the change in CIMTBIF, independent of Framingham Risk Score and baseline CIMTBIF. No correlation was noted between CD8 T-cell activation and CIMTBIF change. Monocyte subsets, CD8 T-cell activation, and biomarker concentrations were not correlated with changes in CIMTCCA. CONCLUSIONS: Our findings highlight the role of non-classical monocytes and MCP-1 in the progression of CIMTBIF in HIV-infected individuals on stable ART independent of traditional cardio-metabolic risk factors.
BACKGROUND:Inflammation may contribute to cardiovascular disease (CVD) among antiretrovirally suppressed HIV-infected individuals. We assessed relationships of monocyte, CD8 T-cell activation and plasma biomarkers to changes in carotid artery intima-media thickness (CIMT). METHODS: Longitudinal study of HIV-infected subjects ≥40 years and on stable antiretroviral therapy (ART) ≥3 months. Peripheral blood mononuclear cells were immunophenotyped by multiparameteric flow cytometry to quantify classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), non-classical (CD14(low/+)CD16(++)) and transitional (CD14(+)CD16(-)) monocyte subsets and activated (CD38(+)HLA-DR(+)) CD8(+) T-cells at baseline. Plasma biomarkers were assessed by multiplex Luminex assay. High-resolution B-mode ultrasounds of right carotid arteries were obtained. Changes in CIMT over two years at the right common carotid artery (CIMTCCA) and right bifurcation (CIMTBIF) were outcome variables. RESULTS: We studied 50 subjects: 84% male, median age 49 (Q1, Q3; 46, 56) years, median CD4 count 461 (317, 578) cells/mm(3), and with HIV RNA ≤ 50 copies/mL in 84%. Change in CIMTBIF correlated with log values of baseline absolute count of non-classical monocytes (r = 0.37, p = 0.020), and with MCP-1 (r = 0.42, p = 0.0024) and TNF-α (r = 0.30, p = 0.036) levels. In multivariable linear regression, only non-classical monocytes and MCP-1 predicted the change in CIMTBIF, independent of Framingham Risk Score and baseline CIMTBIF. No correlation was noted between CD8 T-cell activation and CIMTBIF change. Monocyte subsets, CD8 T-cell activation, and biomarker concentrations were not correlated with changes in CIMTCCA. CONCLUSIONS: Our findings highlight the role of non-classical monocytes and MCP-1 in the progression of CIMTBIF in HIV-infected individuals on stable ART independent of traditional cardio-metabolic risk factors.
Authors: Jason D Barbour; Emilie C Jalbert; Dominic C Chow; Louie Mar A Gangcuangco; Philip J Norris; Sheila M Keating; John Heitman; Lorna Nagamine; Todd Seto; Lishomwa C Ndhlovu; Beau K Nakamoto; Howard N Hodis; Nisha I Parikh; Cecilia M Shikuma Journal: Atherosclerosis Date: 2013-10-31 Impact factor: 5.162
Authors: Matthew S Freiberg; Chung-Chou H Chang; Lewis H Kuller; Melissa Skanderson; Elliott Lowy; Kevin L Kraemer; Adeel A Butt; Matthew Bidwell Goetz; David Leaf; Kris Ann Oursler; David Rimland; Maria Rodriguez Barradas; Sheldon Brown; Cynthia Gibert; Kathy McGinnis; Kristina Crothers; Jason Sico; Heidi Crane; Alberta Warner; Stephen Gottlieb; John Gottdiener; Russell P Tracy; Matthew Budoff; Courtney Watson; Kaku A Armah; Donna Doebler; Kendall Bryant; Amy C Justice Journal: JAMA Intern Med Date: 2013-04-22 Impact factor: 21.873
Authors: Julie A Womack; Chung-Chou H Chang; Kaku A So-Armah; Charles Alcorn; Jason V Baker; Sheldon T Brown; Matthew Budoff; Adeel A Butt; Cynthia Gibert; Matthew Bidwell Goetz; John Gottdiener; Stephen Gottlieb; Amy C Justice; David Leaf; Kathleen McGinnis; David Rimland; Maria C Rodriguez-Barradas; Jason Sico; Melissa Skanderson; Hilary Tindle; Russell P Tracy; Alberta Warner; Matthew S Freiberg Journal: J Am Heart Assoc Date: 2014-10-16 Impact factor: 5.501
Authors: Emilie Jalbert; Timothy Q Crawford; Michelle L D'Antoni; Sheila M Keating; Philip J Norris; Beau K Nakamoto; Todd Seto; Nisha I Parikh; Cecilia M Shikuma; Lishomwa C Ndhlovu; Jason D Barbour Journal: PLoS One Date: 2013-09-25 Impact factor: 3.240
Authors: Cecilia M Shikuma; Dominic C Chow; Louie Mar A Gangcuangco; Guangxiang Zhang; Sheila M Keating; Philip J Norris; Todd B Seto; Nisha Parikh; Kalpana J Kallianpur; Beau K Nakamoto; Lorna S Nagamine; Lishomwa C Ndhlovu; Jason D Barbour Journal: PLoS One Date: 2014-02-27 Impact factor: 3.240
Authors: Dominic C Chow; Makoa Mau; Howard N Hodis; Jakrin Kewcharoen; Yanjie Li; Chathura Siriwardhana; Scott A Souza; Brooks I Mitchell; Scott Bowler; Ivo SahBandar; Louie Mar A Gangcuangco; Iain MacPherson; Lishomwa C Ndhlovu; Cecilia M Shikuma Journal: AIDS Res Hum Retroviruses Date: 2020-09-28 Impact factor: 2.205
Authors: Karin A L Mueller; David B Hanna; Erik Ehinger; Xiaonan Xue; Livia Baas; Meinrad P Gawaz; Tobias Geisler; Kathryn Anastos; Mardge H Cohen; Stephen J Gange; Sonya L Heath; Jason M Lazar; Chenglong Liu; Wendy J Mack; Igho Ofotokun; Phyllis C Tien; Howard N Hodis; Alan L Landay; Robert C Kaplan; Klaus Ley Journal: Cardiovasc Res Date: 2019-05-01 Impact factor: 13.081
Authors: Lishomwa C Ndhlovu; Philip J Norris; Erika G Marques de Menezes; Xutao Deng; Jocelyn Liu; Scott A Bowler; Cecilia M Shikuma; Mars Stone; Peter W Hunt Journal: mBio Date: 2022-04-18 Impact factor: 7.786
Authors: Rafael T Mattos; Nayara I Medeiros; Carlos A Menezes; Rafaelle C G Fares; Eliza P Franco; Walderez O Dutra; Fabrício Rios-Santos; Rodrigo Correa-Oliveira; Juliana A S Gomes Journal: PLoS One Date: 2016-12-15 Impact factor: 3.240
Authors: Ivo N SahBandar; Lishomwa C Ndhlovu; Katelyn Saiki; Lindsay B Kohorn; Mary Margaret Peterson; Michelle L D'Antoni; Bruce Shiramizu; Cecilia M Shikuma; Dominic C Chow Journal: J Atheroscler Thromb Date: 2019-10-04 Impact factor: 4.928