Song-Nian Liang1, Ke Xu1, Hong-Shan Zhong2. 1. Department of Radiology, The First Affiliated Hospital of China Medical University; 2. The Key Laboratory of Imaging Diagnosis and Interventional Treatment of Liaoning Province, Shenyang, Liaoning Province 110001, P.R. China.
Abstract
BACKGROUND: The aim of this study was to evaluate the feasibility of a high fat diet (HFD) associated with pancreatic elastase (PE) infiltration, in establishing the rabbit aortic atherosclerosis model. METHODS: The HFD+PE method and the HFD+saccule injury (SI) method were simultaneously used to prepare the rabbit atherosclerosis model; the control group was established with the normal diet. Biochemical indicators, radiological imaging, pathomorphology and immunohistochemistry were used to evaluate the HFD+PE modeling results. RESULTS: There were significant changes in the blood lipid contents, as well as the pathomorphological and immunohistochemical results between the two experimental groups and the control group (p < 0.05). However, there was no difference between the two experimental groups. The rabbit aortic atherosclerosis model prepared by the HFD+PE method had no significant difference in the local vascular pathomorphological and immunohistochemical results with the traditional HFD+SI method. CONCLUSIONS: The use of HFD with PE infiltration is feasible in establishing the rabbit aortic atherosclerosis model. KEY WORDS: Animal model; Atherosclerosis; Rabbit.
BACKGROUND: The aim of this study was to evaluate the feasibility of a high fat diet (HFD) associated with pancreatic elastase (PE) infiltration, in establishing the rabbit aortic atherosclerosis model. METHODS: The HFD+PE method and the HFD+saccule injury (SI) method were simultaneously used to prepare the rabbit atherosclerosis model; the control group was established with the normal diet. Biochemical indicators, radiological imaging, pathomorphology and immunohistochemistry were used to evaluate the HFD+PE modeling results. RESULTS: There were significant changes in the blood lipid contents, as well as the pathomorphological and immunohistochemical results between the two experimental groups and the control group (p < 0.05). However, there was no difference between the two experimental groups. The rabbit aortic atherosclerosis model prepared by the HFD+PE method had no significant difference in the local vascular pathomorphological and immunohistochemical results with the traditional HFD+SI method. CONCLUSIONS: The use of HFD with PE infiltration is feasible in establishing the rabbit aortic atherosclerosis model. KEY WORDS: Animal model; Atherosclerosis; Rabbit.
Authors: Claudio Vinegoni; Ion Botnaru; Elena Aikawa; Marcella A Calfon; Yoshiko Iwamoto; Eduardo J Folco; Vasilis Ntziachristos; Ralph Weissleder; Peter Libby; Farouc A Jaffer Journal: Sci Transl Med Date: 2011-05-25 Impact factor: 17.956
Authors: N S van Ditzhuijzen; M van den Heuvel; O Sorop; R W B van Duin; I Krabbendam-Peters; R van Haeren; J M R Ligthart; K T Witberg; D J Duncker; E Regar; H M M van Beusekom; W J van der Giessen Journal: Neth Heart J Date: 2011-10 Impact factor: 2.380