Literature DB >> 2712285

An inexpensive small volume equilibrium dialysis system for protein-ligand binding assays.

T Reinard1, H J Jacobsen.   

Abstract

A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

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Year:  1989        PMID： 2712285     DOI： 10.1016/0003-2697(89)90286-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  5'-Azido-[3,6-3H2]-1-napthylphthalamic acid, a photoactivatable probe for naphthylphthalamic acid receptor proteins from higher plants: identification of a 23-kDa protein from maize coleoptile plasma membranes.

Authors:  R Zettl; J Feldwisch; W Boland; J Schell; K Palme
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

2.  A soluble auxin-binding protein from Hyoscyamus muticus is a glutathione S-transferase.

Authors:  J Bilang; H Macdonald; P J King; A Sturm
Journal:  Plant Physiol       Date:  1993-05       Impact factor: 8.340

3.  Influence of gestational age and body weight on the pharmacokinetics of labetalol in pregnancy.

Authors:  James H Fischer; Gloria E Sarto; Jennifer Hardman; Loraine Endres; Thomas M Jenkins; Sarah J Kilpatrick; Hyunyoung Jeong; Stacie Geller; Kelly Deyo; Patricia A Fischer; Keith A Rodvold
Journal:  Clin Pharmacokinet       Date:  2014-04       Impact factor: 6.447

4.  Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis.

Authors:  L Rey; J Imperial; J M Palacios; T Ruiz-Argüeso
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490

5.  Protein binding modulates inhibition of the epidermal growth factor receptor kinase and DNA synthesis by tyrphostins.

Authors:  R Hoffman; I F Dennis; J Donaldson
Journal:  Cancer Chemother Pharmacol       Date:  1995       Impact factor: 3.333

6.  Molecular cloning and structural analysis of a gene from Zea mays (L.) coding for a putative receptor for the plant hormone auxin.

Authors:  T Hesse; J Feldwisch; D Balshüsemann; G Bauw; M Puype; J Vandekerckhove; M Löbler; D Klämbt; J Schell; K Palme
Journal:  EMBO J       Date:  1989-09       Impact factor: 11.598
  6 in total

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