| Literature DB >> 27117659 |
Abstract
In clinical metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) has become a very useful technique for the analysis of highly polar and charged metabolites in complex biologic samples. A comprehensive overview of recent developments in CE-MS for metabolic profiling studies is presented. This review covers theory, CE separation modes, capillary coatings, and practical aspects of CE-MS coupling. Attention is also given to sample pretreatment and data analysis strategies used for metabolomics. The applicability of CE-MS for clinical metabolomics is illustrated using samples ranging from plasma and urine to cells and tissues. CE-MS application to large-scale and quantitative clinical metabolomics is addressed. Conclusions and perspectives on this unique analytic strategy are presented.Keywords: Blood; Capillary electrophoresis; Cerebrospinal fluid; Data analysis; Mass spectrometry; Metabolomics; Sample pretreatment; Urine
Mesh：
Year: 2016 PMID： 27117659 DOI： 10.1016/bs.acc.2015.12.002
Source DB: PubMed Journal: Adv Clin Chem ISSN： 0065-2423 Impact factor: 5.394