Literature DB >> 27117176

Labeling of target mRNAs using a photo-reactive microRNA probe.

Kosuke Nakamoto1, Koichiro Minami2, Yukihiro Akao2, Yoshihito Ueno3.   

Abstract

To identify target mRNAs of an miRNA, we synthesized photo-reactive miRNA probes, which contained a photo-reactive nucleoside analog, 1-O-[4-(3-trifluoromethyl-3H-diazirine-3-yl)]benzyl-β-d-ribofuranose, in the middle of the strand. The photo-reactive miRNA-145 probe was found to specifically label the target mRNAs, FSCN1 and KLF4, by UV-A irradiation in human colon cancer DLD-1 cells.

Entities:  

Mesh：

Substances：

Year:  2016        PMID： 27117176     DOI： 10.1039/c6cc01360k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  3 in total

Review 1.  Current advances of carbene-mediated photoaffinity labeling in medicinal chemistry.

Authors:  Sha-Sha Ge; Biao Chen; Yuan-Yuan Wu; Qing-Su Long; Yong-Liang Zhao; Pei-Yi Wang; Song Yang
Journal:  RSC Adv       Date:  2018-08-20       Impact factor: 4.036

2.  Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes.

Authors:  Zhiyu Su; Tsogzolmaa Ganbold; Huricha Baigude
Journal:  Int J Mol Sci       Date:  2020-02-24       Impact factor: 5.923

3.  A Genetically Encoded Diazirine Analogue for RNA-Protein Photo-crosslinking.

Authors:  Dmytro Dziuba; Jan-Erik Hoffmann; Matthias W Hentze; Carsten Schultz
Journal:  Chembiochem       Date:  2019-11-26       Impact factor: 3.164
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.