| Literature DB >> 27116606 |
Beatriz Sánchez-Calvo, Adriana Cassina, Natalia Rios, Gonzalo Peluffo, José Boggia, Rafael Radi, Homero Rubbo, Andrés Trostchansky.
Abstract
[This corrects the article DOI: 10.1371/journal.pone.0150459.].Entities:
Year: 2016 PMID: 27116606 PMCID: PMC4846006 DOI: 10.1371/journal.pone.0154651
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 1ANG II-mediatedO2.-production is inhibited by NO2-AA.
(A) Dehydroethydium (DHE) oxidation as an index of O2.- production in ANG II-stimulated HK-2 cells (1x106 cells) was determined by HPLC with fluorescence detection (λem = 595 nm, λexc = 510 nm). Cells were preincubated 30 min with vehicle, 10 μM NO2-AA or 10 μM AA and then exposed to DHE in simultaneous with 0.1 μM ANG II for 3 h. Quantitative analysis, expressed as the mean fold change than control condition without ANG II stimulation ± SEM, n = 3 of 2-OH-Et+ (B) and Et+ (C) are shown. *, # correspond to significant data relative to control and ANG II-treated cells, respectively (p<0.05).