Efficacy of forming biofilms by Pseudomonas migulae AN-1 toward in situ bioremediation of aniline-contaminated aquifer by groundwater circulation wells.

The formation and activity of aniline-degrading biofilms developed by the psychrotrophic Pseudomonas migulae AN-1 were studied for the in situ remediation of contaminated aquifer using in-well bioreactor of groundwater circulating wells (GCWs). Biofilms grown in mineral salt medium with aniline exhibited tolerance to high concentrations of aniline. In aniline degradation rate, AN-1 biofilms exhibited slight differences compared with planktonic cells. The effectiveness and bio-implication of AN-1 biofilms in GCWs were investigated to treat aniline-contaminated aquifer. The results demonstrate that AN-1 biofilms survived the GCWs treatment process with high aniline-degrading efficiency. This system provides a novel environmentally friendly technology for the in situ bioremediation of low-volatile contaminants.