Ryo Hoshikawa1, Hiroshi Kawaguchi2, Hiroyuki Takuwa3, Yoko Ikoma3, Yutaka Tomita4, Miyuki Unekawa4, Norihiro Suzuki4, Iwao Kanno3, Kazuto Masamoto1,3,5. 1. Faculty of Informatics and Engineering, University of Electro-Communications, Tokyo, Japan. 2. Human Informatics Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan. 3. Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan. 4. Department of Neurology, Keio University School of Medicine, Tokyo, Japan. 5. Brain Science Inspired Life Support Research Center, University of Electro-Communications, Tokyo, Japan.
Abstract
OBJECTIVE: This study aimed to develop a new method for mapping blood flow velocity based on the spatial evolution of fluorescent dye transit times captured with CLSFM in the cerebral microcirculation of anesthetized rodents. METHODS: The animals were anesthetized with isoflurane, and a small amount of fluorescent dye was intravenously injected to label blood plasma. The CLSFM was conducted through a closed cranial window to capture propagation of the dye in the cortical vessels. The transit time of the dye over a certain distance in a single vessel was determined with automated image analyses, and average flow velocity was mapped in each vessel. RESULTS: The average flow velocity measured in the rat pial artery and vein was 4.4 ± 1.2 and 2.4 ± 0.5 mm/sec, respectively. A similar range of flow velocity to those of the rats was observed in the mice; 4.9 ± 1.4 and 2.0 ± 0.9 mm/sec, respectively, although the vessel diameter in the mice was about half of that in the rats. CONCLUSIONS: Flow velocity in the cerebral microcirculation can be mapped based on fluorescent dye transit time measurements with conventional CLSFM in experimental animals.
OBJECTIVE: This study aimed to develop a new method for mapping blood flow velocity based on the spatial evolution of fluorescent dye transit times captured with CLSFM in the cerebral microcirculation of anesthetized rodents. METHODS: The animals were anesthetized with isoflurane, and a small amount of fluorescent dye was intravenously injected to label blood plasma. The CLSFM was conducted through a closed cranial window to capture propagation of the dye in the cortical vessels. The transit time of the dye over a certain distance in a single vessel was determined with automated image analyses, and average flow velocity was mapped in each vessel. RESULTS: The average flow velocity measured in the rat pial artery and vein was 4.4 ± 1.2 and 2.4 ± 0.5 mm/sec, respectively. A similar range of flow velocity to those of the rats was observed in the mice; 4.9 ± 1.4 and 2.0 ± 0.9 mm/sec, respectively, although the vessel diameter in the mice was about half of that in the rats. CONCLUSIONS: Flow velocity in the cerebral microcirculation can be mapped based on fluorescent dye transit time measurements with conventional CLSFM in experimental animals.
Authors: Sherry G Clendenon; Xiao Fu; Robert A Von Hoene; Jeffrey L Clendenon; James P Sluka; Seth Winfree; Henry Mang; Michelle Martinez; Adele J Filson; James E Klaunig; James A Glazier; Kenneth W Dunn Journal: Microvasc Res Date: 2018-11-28 Impact factor: 3.514