Oznur Sen1, Nevzat Cem Sayilgan2, Ayse Cigdem Tutuncu2, Mefkur Bakan3, Guniz Meyanci Koksal2, Huseyin Oz4. 1. Department of Anesthesiology and Reanimation, Ministry of Health, Haseki Training and Research Hospital, Istanbul, Turkey. Electronic address: senoznur@gmail.com. 2. Department of Anesthesiology and Reanimation, Istanbul University, Cerrahpasa Medical Faculty, Istanbul, Turkey. 3. Department of Anesthesiology and Reanimation, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Turkey. 4. Department of Anesthesiology and Reanimation, Medipol University, Faculty of Medicine, Istanbul, Turkey.
Abstract
OBJECTIVE: The local anesthetics may cause neurotoxicity. We aimed to compare the neurotoxic potential of different local anesthetics, local anesthetic induced nerve damage and pathological changes of a peripheral nerve. METHODS: Sixty Wistar rats weighing 200-350g were studied. Rats were assigned into 3 groups and 26-gauge needle was inserted under magnification into the left sciatic nerve and 0.2mL of 0.5% bupivacaine, 5% levobupivacaine, and 2% lidocaine were injected intraneurally. An individual who was blind to the specifics of the injection monitored the neurologic function on postoperative 1st day, and daily thereafter. Neurologic examination included assessment for the presence and severity of nociception and grasping reflexes. At the 7th day sciatic nerve specimen was taken for evaluation of histopathologic changes. RESULTS: There was no statistical difference detected among groups regarding grasping reflex and histopathologic evaluation. Two cases in bupivacaine group, 1 case in levobupivacaine group and 2 cases in lidocaine group had slight grasping, while 1 case in lidocaine group had no grasping reflex on the seventh day. Severe axonal degeneration was observed in all groups, respectively in bupivacaine group 4 (20%), levobupivacaine group 3 (15%), and lidocaine group 6 (30%). CONCLUSION: In all groups, histopathological damage frequency and severity were more than the motor deficiency.
OBJECTIVE: The local anesthetics may cause neurotoxicity. We aimed to compare the neurotoxic potential of different local anesthetics, local anesthetic induced nerve damage and pathological changes of a peripheral nerve. METHODS: Sixty Wistar rats weighing 200-350g were studied. Rats were assigned into 3 groups and 26-gauge needle was inserted under magnification into the left sciatic nerve and 0.2mL of 0.5% bupivacaine, 5% levobupivacaine, and 2% lidocaine were injected intraneurally. An individual who was blind to the specifics of the injection monitored the neurologic function on postoperative 1st day, and daily thereafter. Neurologic examination included assessment for the presence and severity of nociception and grasping reflexes. At the 7th day sciatic nerve specimen was taken for evaluation of histopathologic changes. RESULTS: There was no statistical difference detected among groups regarding grasping reflex and histopathologic evaluation. Two cases in bupivacaine group, 1 case in levobupivacaine group and 2 cases in lidocaine group had slight grasping, while 1 case in lidocaine group had no grasping reflex on the seventh day. Severe axonal degeneration was observed in all groups, respectively in bupivacaine group 4 (20%), levobupivacaine group 3 (15%), and lidocaine group 6 (30%). CONCLUSION: In all groups, histopathological damage frequency and severity were more than the motor deficiency.
Authors: Mansour Sobeh; Mona F Mahmoud; Samar Rezq; Mohamed A O Abdelfattah; Islam Mostafa; Amira E Alsemeh; Assem M El-Shazly; Aziz Yasri; Michael Wink Journal: Biomolecules Date: 2020-03-02
Authors: Mansour Sobeh; Mona F Mahmoud; Samar Rezq; Amira E Alsemeh; Omar M Sabry; Islam Mostafa; Mohamed A O Abdelfattah; Khadija Ait El-Allem; Assem M El-Shazly; Aziz Yasri; Michael Wink Journal: Antioxidants (Basel) Date: 2019-10-12