| Literature DB >> 27108310 |
Heloisa S Pitz1, Adriana C D Trevisan1, Fausto R Cardoso1, Aline Pereira1, Eduardo L G Moreira2, Manuel A de Prá1,2, Letícia Mazzarino1,2, Maria B Veleirinho1,2, Rosendo A Yunes1, Rosa M R do Valle1, Marcelo Maraschin3.
Abstract
Plinia cauliflora (jaboticaba) is a native fruit tree from Brazilian rainforest widely used in popular medicine to prevent diarrhea, asthma, and infections. Studies have shown that the major therapeutic potential of jaboticaba fruits is on its peel, a rich source of anthocyanins. These secondary metabolites have well-known antioxidant and anti-inflammatory activities and have been claimed to be effective to treat diabetes, cancer, cardiovascular diseases, and stroke. This chapter describes a series of methodologies to evaluate important in vitro biological activities like cytotoxicity, proliferation, and migration of a hydroalcoholic extract of jaboticaba peel on mouse fibroblast L929 line. Assays to assess total phenolic, flavonoid, and anthocyanin contents and antioxidant activities are described as well.Entities:
Keywords: Anthocyanins; Biological activities; DPPH assay; EdU assay; MTT assay; Phenolic compounds; Phytochemistry; Plinia cauliflora; Scratch assay; Total flavonoids
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Year: 2016 PMID： 27108310 DOI： 10.1007/978-1-4939-3332-7_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745