Imran Ullah1, Raghavendra Baregundi Subbarao1, Eun-Jin Kim2, Dinesh Bharti1, Si-Jung Jang1, Ji-Sung Park1, Sharath Belame Shivakumar1, Sung-Lim Lee1, Dawon Kang2, June-Ho Byun3, Bong-Wook Park4, Gyu-Jin Rho5. 1. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea. 2. Department of Physiology and Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-751, Republic of Korea. 3. Department of Oral and Maxillofacial Surgery, Institute of Health Science, School of Medicine, Gyeongsang National University, Republic of Korea. 4. Department of Oral and Maxillofacial Surgery, Institute of Health Science, School of Medicine, Gyeongsang National University, Republic of Korea. Electronic address: parkbw@gnu.ac.kr. 5. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: jinrho@gnu.ac.kr.
Abstract
AIMS: The aim of this study was to find out a mesenchymal stem cells (MSCs) source from human dental tissues of the same donor (follicle, papilla and pulp), which exhibits higher neurogenic differentiation potential in vitro. MAIN METHODS: MSCs were isolated from dental tissues (follicle, papilla and pulp) by digestion method. All MSCs were analyzed for pluripotent makers by western blot, cell surface markers by flow cytometry, adipo- and osteocytes markers by RT-qPCR. The neuronal differentiated MSCs were characterized for neuronal specific markers by RT-qPCR and immunofluorescence. Functional neuronal properties were analyzed by electrophysiology and synaptic markers expression. KEY FINDINGS: All MSCs expressed pluripotent markers (Oct4, Sox2 and Nanog) and were found positive for mesenymal markers (CD44, CD90, CD105) while negative for hematopoietic markers (CD34 and CD45). Furthermore, MSCs were successfully differentiated into adipocytes, osteocytes and trans-differentiated into neuronal cells. Among them, dental pulp derived MSCs exhibits higher neurogenic differentiation potential, in term of expression of neuronal specific markers at both gene and protein level, and having higher Na(+) and K(+) current with the expression of synaptic markers. SIGNIFICANCE: The three types of dental MSCs from a single donor broadly possessed similar cellular properties and can differentiate into neuronal cells; however, pulp derived MSCs showed higher neurogenic potential than the follicle and papilla, suggesting their use in future stem cells therapy for the treatment of neurodegenerative disorders.
AIMS: The aim of this study was to find out a mesenchymal stem cells (MSCs) source from human dental tissues of the same donor (follicle, papilla and pulp), which exhibits higher neurogenic differentiation potential in vitro. MAIN METHODS: MSCs were isolated from dental tissues (follicle, papilla and pulp) by digestion method. All MSCs were analyzed for pluripotent makers by western blot, cell surface markers by flow cytometry, adipo- and osteocytes markers by RT-qPCR. The neuronal differentiated MSCs were characterized for neuronal specific markers by RT-qPCR and immunofluorescence. Functional neuronal properties were analyzed by electrophysiology and synaptic markers expression. KEY FINDINGS: All MSCs expressed pluripotent markers (Oct4, Sox2 and Nanog) and were found positive for mesenymal markers (CD44, CD90, CD105) while negative for hematopoietic markers (CD34 and CD45). Furthermore, MSCs were successfully differentiated into adipocytes, osteocytes and trans-differentiated into neuronal cells. Among them, dental pulp derived MSCs exhibits higher neurogenic differentiation potential, in term of expression of neuronal specific markers at both gene and protein level, and having higher Na(+) and K(+) current with the expression of synaptic markers. SIGNIFICANCE: The three types of dental MSCs from a single donor broadly possessed similar cellular properties and can differentiate into neuronal cells; however, pulp derived MSCs showed higher neurogenic potential than the follicle and papilla, suggesting their use in future stem cells therapy for the treatment of neurodegenerative disorders.
Authors: Meer N Ahmed; Delin Shi; Matthew T Dailey; Kristi Rothermund; Michelle D Drewry; Tia C Calabrese; Xinyan T Cui; Fatima N Syed-Picard Journal: Tissue Eng Part A Date: 2020-12-21 Impact factor: 4.080
Authors: Alicia L Bertone; Nathalie A Reisbig; Allison H Kilborne; Mari Kaido; Navid Salmanzadeh; Rebecca Lovasz; Joy L Sizemore; Logan Scheuermann; Rosalind J Kopp; Lisa J Zekas; Matthew T Brokken Journal: Front Vet Sci Date: 2017-03-10
Authors: Letícia Fracaro; Alexandra C Senegaglia; Roberto H Herai; Amanda Leitolis; Lidiane M Boldrini-Leite; Carmen L K Rebelatto; Paul J Travers; Paulo R S Brofman; Alejandro Correa Journal: Int J Mol Sci Date: 2020-04-15 Impact factor: 5.923