| Literature DB >> 27107383 |
Jing Zhang1, Yujie Sun1, Baocheng Tian1, Keke Li1, Lele Wang1, Yan Liang1, Jingtian Han2.
Abstract
In this paper, a CD44-targeted and redox-responsive drug delivery system based on mesoporous silica nanoparticles (MSNs) was synthesized by conjugating tumor-shedable hyaluronic acid (HA) on the surface of MSNs via disulfide bonds. Doxorubicin hydrochloride (DOX·HCl) was physically encapsulated into HA modified MSNs (MSNs/SS/HA@DOX) as a model drug. MSNs/SS/HA@DOX (40nm) had a high drug loading (14.1%) and redox-responsive drug release property. The cellular uptake behaviors of MSNs/SS/HA@DOX by HeLa and LO2 cells were evaluated by confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). MSNs/SS/HA@DOX exhibited higher cellular uptake efficacy via CD44-mediated endocytosis by HeLa cells (CD44 over-expressed cells) than by LO2 cells (CD44 deficient cells). The in vitro cytotoxicity assay demonstrated that MSNs/SS/HA@DOX exhibited higher cytotoxicity to HeLa cells than to LO2 cells. These results indicated that MSNs/SS/HA@DOX might be promising as a multifunctional drug delivery system to improve the anti-tumor efficacy of chemotherapeutic drugs.Entities:
Keywords: CD44-targeted; Doxorubicin; Drug delivery system; Mesoporous silica nanoparticles; Redox-responsive
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Year: 2016 PMID： 27107383 DOI： 10.1016/j.colsurfb.2016.04.015
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268