Cândida Abreu1, André Silva-Pinto2, Daniela Lazzara3, Joana Sobrinho-Simões3, João Tiago Guimarães4, António Sarmento5. 1. Department of Infectious Diseases, Centro Hospitalar S. João, Porto, Portugal; Instituto de Inovação e Investigação em Saúde (I3S), Grupo de I&D em Nefrologia e Doenças Infecciosas, Instituto Nacional de Engenharia Biomédica (INEB), Portugal. Electronic address: candida.abreu@gmail.com. 2. Department of Infectious Diseases, Centro Hospitalar S. João, Porto, Portugal. 3. Clinical Pathology Service, Centro Hospitalar S. João, Portugal. 4. Clinical Pathology Service, Centro Hospitalar S. João, Portugal; EPIUnit, Institute of Public Health, University of Porto, Portugal. 5. Department of Infectious Diseases, Centro Hospitalar S. João, Porto, Portugal; Instituto de Inovação e Investigação em Saúde (I3S), Grupo de I&D em Nefrologia e Doenças Infecciosas, Instituto Nacional de Engenharia Biomédica (INEB), Portugal.
Abstract
BACKGROUND: All the reports from Angola's 2013 dengue outbreak revealed serotype 1. However, previously dengue serotypes 1-4 have been reported in Africa and in 2014 serotype 4 was reported in Angola. OBJECTIVES: To report dengue serotypes in patients returning from Angola during 2013 outbreak. STUDY DESIGN: Retrospective, cross-sectional study. We serotyped the dengue by an in house Polymerase Chain Reaction technique in randomly selected cases. RESULTS: From the 2013 Angola's dengue outbreak we treated 47 adult patients. None had history of past dengue. A combo kit test for dengue revealed positive NS1 antigen in 39 and IgM antibodies in 8. From 17 randomly patients tested by RNA Real Time-PCR, 11 were positive: 7 for DENV-1, 2 for DENV-2, 1 for DENV-3 (co-infected with DENV-1) and 1 for DENV-4. None had a complicated or fatal evolution. CONCLUSION: Unlike previous reports the 4 serotypes were detected, and this resulted in a different epidemiological situation, raising the risk of future outbreaks of severe dengue.
BACKGROUND: All the reports from Angola's 2013 dengue outbreak revealed serotype 1. However, previously dengue serotypes 1-4 have been reported in Africa and in 2014 serotype 4 was reported in Angola. OBJECTIVES: To report dengue serotypes in patients returning from Angola during 2013 outbreak. STUDY DESIGN: Retrospective, cross-sectional study. We serotyped the dengue by an in house Polymerase Chain Reaction technique in randomly selected cases. RESULTS: From the 2013 Angola's dengue outbreak we treated 47 adult patients. None had history of past dengue. A combo kit test for dengue revealed positive NS1 antigen in 39 and IgM antibodies in 8. From 17 randomly patients tested by RNA Real Time-PCR, 11 were positive: 7 for DENV-1, 2 for DENV-2, 1 for DENV-3 (co-infected with DENV-1) and 1 for DENV-4. None had a complicated or fatal evolution. CONCLUSION: Unlike previous reports the 4 serotypes were detected, and this resulted in a different epidemiological situation, raising the risk of future outbreaks of severe dengue.
Authors: Zoraima Neto; Pedro A Martinez; Sarah C Hill; Domingos Jandondo; Julien Thézé; Marinela Mirandela; Renato Santana Aguiar; Joilson Xavier; Cruz Dos Santos Sebastião; Ana Luísa Micolo Cândido; Filipa Vaz; Gisel Reyes Castro; Joana Paula Paixão; Nicholas J Loman; Philippe Lemey; Oliver G Pybus; Jocelyne Vasconcelos; Nuno Rodrigues Faria; Joana de Morais Journal: PLoS Negl Trop Dis Date: 2022-05-18
Authors: Sarah C Hill; Jocelyne Neto de Vasconcelos; Bernardo Gutierrez Granja; Julien Thézé; Domingos Jandondo; Zoraima Neto; Marinela Mirandela; Cruz Dos Santos Sebastião; Ana Luísa Micolo Cândido; Carina Clemente; Sara Pereira da Silva; Túlio de Oliveira; Oliver G Pybus; Nuno R Faria; Joana Morais Afonso Journal: Emerg Infect Dis Date: 2019-04 Impact factor: 6.883
Authors: Gaspary O Mwanyika; Leonard E G Mboera; Sima Rugarabamu; Baraka Ngingo; Calvin Sindato; Julius J Lutwama; Janusz T Paweska; Gerald Misinzo Journal: Viruses Date: 2021-03-24 Impact factor: 5.048