Literature DB >> 27106153

Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue.

Ying Liu1, Bin Zhang2, S A Ibrahim3, Shuang-Shuang Gao2, Hong Yang2, Wen Huang4.   

Abstract

In this study, we isolated polysaccharides from Flammulina velutipes residue (FVRP) using microwave-assisted extraction and then purified the polysaccharides by column chromatography to yield FVRP-1, FVRP-2 and FVRP-3. The structural characteristics of FVRP-1, FVRP-2 and FVRP-3 were investigated, and their antioxidant activities against ABTS(+), DPPH and hydroxyl radicals were also analyzed in vitro. FVRP-1 was found to be neutral and rich in galactose. However, FVRP-2 and FVRP-3 were acidic polysaccharides and were rich in glucose. The average molecular weight of FVRP-1, FVRP-2 and FVRP-3 were 29,930, 62,290, and 36,310Da, respectively. The glycosyl residue of FVRP-1 was an α-type glycosidic linkage, whereas FVRP-2 and FVRP-3 were β-type glycosidic linkages. We found FVRP-1, FVRP-2 and FVRP-3 had strong potential antioxidant activities in the order of FVRP-1<FVRP-3<FVRP-2. Our results thus suggested that FVRPs might be suitable for use as functional foods and as potential therapeutic agents.
Copyright © 2016 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Antioxidant activity; Characterization; Flammulina velutipes residue; Polysaccharide; Purification

Mesh:

Substances:

Year:  2016        PMID: 27106153     DOI: 10.1016/j.carbpol.2016.03.020

Source DB:  PubMed          Journal:  Carbohydr Polym        ISSN: 0144-8617            Impact factor:   9.381


  16 in total

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