Literature DB >> 27100355

The effects of NONRATT021972 lncRNA siRNA on PC12 neuronal injury mediated by P2X7 receptor after exposure to oxygen-glucose deprivation.

Guilin Li1, Lifang Zou1, Wei Xie2, Shiyao Wen2, Qiuyu Xie2, Yun Gao1, Changshui Xu1, Hong Xu1, Shuangmei Liu1, Shouyu Wang1, Yun Xue1, Bing Wu1, Qiulan Lv1, Mofeng Ying1, Xi Zhang1, Shangdong Liang3.   

Abstract

Adenosine triphosphate (ATP) participates in signal transmission by acting on P2X receptors, and the P2X7 receptor is involved in the pathophysiological changes of ischemic injury. The PC12 cell line is a popular model system to study sympathetic neuronal function. Long noncoding RNAs (lncRNAs) are highly expressed in the nervous system and serve as regulatory RNAs. In this study, the effects of NONRATT021972 lncRNA siRNA on P2X7-mediated PC12 neuronal injury after exposure to oxygen-glucose deprivation (OGD) were investigated. Our results showed that the viability of PC12 cells cultured with OGD or the P2X7 agonist BzATP was significantly decreased. Treatment with NONRATT021972 siRNA reversed the decreased viability of PC12 cells under OGD conditions. The upregulated P2X7 mRNA and protein levels in PC12 cells under OGD conditions or BzATP treatment were significantly decreased when pretreated with NONRATT021972 siRNA. Moreover, NONRATT021972 siRNA treatment effectively suppressed the increase in [Ca(2+)]i induced by OGD or P2X7 agonists (ATP or BzATP) in PC12 cells. Therefore, treatment with NONRATT021972 siRNA may decrease sympathetic neuronal injury induced by ischemia.

Entities:  

Keywords:  Adenosine triphosphate; Intracellular Ca2+; Long noncoding RNA; Oxygen-glucose deprivation; P2X7 receptor; PC12 cell

Mesh：

Substances：

Year:  2016        PMID： 27100355      PMCID： PMC5023628          DOI： 10.1007/s11302-016-9513-8

Source DB:  PubMed          Journal:  Purinergic Signal        ISSN： 1573-9538            Impact factor:   3.765


  48 in total

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Journal:  Inflammation       Date:  2015-02       Impact factor: 4.092

2.  Nerve growth factor-stimulated neuronal differentiation induces changes in P2 receptor expression and nucleotide-stimulated catecholamine release.

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Journal:  J Neurochem       Date:  2007-01-15       Impact factor: 5.372

3.  P2X(7) receptor at the heart of disease.

Authors:  Ei Vasileiou; R M Montero; C M Turner; G Vergoulas
Journal:  Hippokratia       Date:  2010-07       Impact factor: 0.471

4.  Apoptosis mediated by p53 in rat neural AF5 cells following treatment with hydrogen peroxide and staurosporine.

Authors:  Charlesene McNeill-Blue; Barbara A Wetmore; Joseph F Sanchez; William J Freed; B Alex Merrick
Journal:  Brain Res       Date:  2006-08-09       Impact factor: 3.252

5.  Increased sympathoexcitatory reflex induced by myocardial ischemic nociceptive signaling via P2X2/3 receptor in rat superior cervical ganglia.

Authors:  Guilin Li; Shuangmei Liu; Jun Zhang; Kehua Yu; Changshui Xu; Jiari Lin; Xin Li; Shangdong Liang
Journal:  Neurochem Int       Date:  2010-04-18       Impact factor: 3.921

6.  P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells.

Authors:  G Arslan; C M Filipeanu; E Irenius; B Kull; E Clementi; C Allgaier; D Erlinge; B B Fredholm
Journal:  Neuropharmacology       Date:  2000-01-28       Impact factor: 5.250

7.  Development of an ischemic tolerance model in a PC12 cell line.

Authors:  Joëlle A Hillion; Kenzo Takahashi; Dragan Maric; Christl Ruetzler; Jeffery L Barker; John M Hallenbeck
Journal:  J Cereb Blood Flow Metab       Date:  2005-02       Impact factor: 6.200

8.  Electrophysiological studies of upregulated P2X7 receptors in rat superior cervical ganglia after myocardial ischemic injury.

Authors:  Fanjun Kong; Shuangmei Liu; Changshui Xu; Jun Liu; Guodong Li; Guilin Li; Yun Gao; Hong Lin; Guihua Tu; Haiying Peng; Shuyi Qiu; Bo Fan; Qicheng Zhu; Shicheng Yu; Chaoran Zheng; Shangdong Liang
Journal:  Neurochem Int       Date:  2013-06-14       Impact factor: 3.921

9.  P2X(7) inhibition in stellate ganglia prevents the increased sympathoexcitatory reflex via sensory-sympathetic coupling induced by myocardial ischemic injury.

Authors:  Guihua Tu; Guilin Li; Haiying Peng; Jiang Hu; Jun Liu; Fanjun Kong; Shuangmei Liu; Yun Gao; Changshui Xu; Xiaoling Xu; Shuyi Qiu; Bo Fan; Qicheng Zhu; Shicheng Yu; Chaoran Zheng; Bing Wu; Lichao Peng; Miaomiao Song; Qin Wu; Shangdong Liang
Journal:  Brain Res Bull       Date:  2013-05-17       Impact factor: 4.077

10.  P2 receptors in cardiovascular regulation and disease.

Authors:  David Erlinge; Geoffrey Burnstock
Journal:  Purinergic Signal       Date:  2007-09-21       Impact factor: 3.765
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  2 in total

1.  Long Non-Coding RNA SNHG7 Alleviates Oxygen and Glucose Deprivation/Reoxygenation-Induced Neuronal Injury by Modulating miR-9/SIRT1 Axis in PC12 Cells: Potential Role in Ischemic Stroke.

Authors:  Tao Zhou; Shuai Wang; Kai Lu; Chunhui Yin
Journal:  Neuropsychiatr Dis Treat       Date:  2020-11-24       Impact factor: 2.570

Review 2.  Current Status of Long Non-Coding RNAs in Human Breast Cancer.

Authors:  Stefanie Cerk; Daniela Schwarzenbacher; Jan Basri Adiprasito; Michael Stotz; Georg C Hutterer; Armin Gerger; Hui Ling; George Adrian Calin; Martin Pichler
Journal:  Int J Mol Sci       Date:  2016-09-06       Impact factor: 5.923
  2 in total

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