Literature DB >> 27089245

2D gel-based Proteomics: there's life in the old dog yet.

Reiner Westermeier1.   

Abstract

In the nowadays mainly mass spectrometry-driven world of Proteomics, one can frequently read and hear that 2D gel-based electrophoresis methods are no longer used for proteome analysis. This short review is a compilation of facts why the gel-based workflow is not dead.

Entities:  

Keywords:  SDS polyacrylamide gel electrophoresis; Two-dimensional electrophoresis; gel-based separation methods; isoelectric focusing; mass spectrometry; proteomics

Mesh：

Substances：

Year:  2016        PMID： 27089245     DOI： 10.1080/13813455.2016.1179766

Source DB:  PubMed          Journal:  Arch Physiol Biochem        ISSN： 1381-3455            Impact factor:   4.076


  5 in total

1.  Proteomic analysis of six- and twelve-month hippocampus and cerebellum in a murine Down syndrome model.

Authors:  Guido N Vacano; David S Gibson; Abdullah Arif Turjoman; Jeremy W Gawryluk; Jonathan D Geiger; Mark Duncan; David Patterson
Journal:  Neurobiol Aging       Date:  2017-11-26       Impact factor: 4.673

Review 2.  Isoelectric Point Separations of Peptides and Proteins.

Authors:  Melissa R Pergande; Stephanie M Cologna
Journal:  Proteomes       Date:  2017-01-25

Review 3.  Characterization of Contractile Proteins from Skeletal Muscle Using Gel-Based Top-Down Proteomics.

Authors:  Paul Dowling; Margit Zweyer; Dieter Swandulla; Kay Ohlendieck
Journal:  Proteomes       Date:  2019-06-20

4.  Alterations of the Platelet Proteome in Lung Cancer: Accelerated F13A1 and ER Processing as New Actors in Hypercoagulability.

Authors:  Huriye Ercan; Lisa-Marie Mauracher; Ella Grilz; Lena Hell; Roland Hellinger; Johannes A Schmid; Florian Moik; Cihan Ay; Ingrid Pabinger; Maria Zellner
Journal:  Cancers (Basel)       Date:  2021-05-08       Impact factor: 6.639

5.  Comparative proteomics reveals unexpected quantitative phosphorylation differences linked to platelet activation state.

Authors:  G J Schmidt; C M Reumiller; H Ercan; U Resch; E Butt; S Heber; Z Liutkevičiūte; J Basílio; J A Schmid; A Assinger; B Jilma; M Zellner
Journal:  Sci Rep       Date:  2019-12-12       Impact factor: 4.379
  5 in total

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