Éva Brigitta Patay1, Nikolett Sali2, Tamás Kőszegi2, Rita Csepregi1, Viktória Lilla Balázs1, Tibor Sebastian Németh3, Tibor Németh3, Nóra Papp4. 1. Institute of Pharmacognosy, Medical School, University of Pécs, Rókus 2, Pécs, 7624, Hungary. 2. Department of Laboratory Medicine, Medical School, University of Pécs, Ifjúság 13, Pécs, 7624, Hungary; János Szentágothai Research Center, Ifjúság 20, Pécs, 7624, Hungary. 3. Department of Pharmacognosy, Faculty of Medicine and Pharmacy, University of Oradea, Piaţa 1 Decembrie u. 10, Oradea, 410073, Romania. 4. Institute of Pharmacognosy, Medical School, University of Pécs, Rókus 2, Pécs, 7624, Hungary. Electronic address: nora4595@gamma.ttk.pte.hu.
Abstract
OBJECTIVE: To investigate the antioxidant activity, total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis (C. benghalensis) and Coffea liberica compared to Coffea arabica (C. arabica). METHODS: The antioxidant potential, total tannin and polyphenol contents of the immature and mature seed and pericarp of C. benghalensis and Coffea liberica were quantified and compared to C. arabica. Enhanced chemiluminescence (ECL), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity, Folin-Ciocalteau method and total tannin content assays were used. RESULTS: Trolox equivalent (TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation (r(2) = 0.587) while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant. A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique (r(2) = 0.610 7) in each species, however the immature pericarp of C. benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay. The immature pericarp of C. benghalensis expressed the highest tannin and polyphenol content, and a high polyphenol level was also detected in the immature seed of C. arabica. The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents. CONCLUSIONS: The obtained data highlight the potential role of C. benghalensis as a new source of natural antioxidants and polyphenols compared to C. arabica.
OBJECTIVE: To investigate the antioxidant activity, total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis (C. benghalensis) and Coffea liberica compared to Coffea arabica (C. arabica). METHODS: The antioxidant potential, total tannin and polyphenol contents of the immature and mature seed and pericarp of C. benghalensis and Coffea liberica were quantified and compared to C. arabica. Enhanced chemiluminescence (ECL), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity, Folin-Ciocalteau method and total tannin content assays were used. RESULTS:Trolox equivalent (TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation (r(2) = 0.587) while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant. A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique (r(2) = 0.610 7) in each species, however the immature pericarp of C. benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay. The immature pericarp of C. benghalensis expressed the highest tannin and polyphenol content, and a high polyphenol level was also detected in the immature seed of C. arabica. The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents. CONCLUSIONS: The obtained data highlight the potential role of C. benghalensis as a new source of natural antioxidants and polyphenols compared to C. arabica.
Authors: Marianna Kocsis; Alexandra Bodó; Tamás Kőszegi; Rita Csepregi; Rita Filep; Gyula Hoffmann; Ágnes Farkas Journal: Int J Mol Sci Date: 2022-01-11 Impact factor: 5.923