Trinh Thi Thuy1, Dao Duc Thien2, Tran Quang Hung2, Nguyen Thanh Tam2, Nguyen Thi Hoang Anh2, Nguyen Thi Nga3, Nguyen Thi Cuc3, Le Phuong Mai4, Tran Van Sung2, Domenico V Delfino5, Do Thi Thao6. 1. Institute of Chemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Viet Nam. Electronic address: drthuy2001@yahoo.de. 2. Institute of Chemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Viet Nam. 3. Institute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Viet Nam. 4. National Research Council Canada, Measurement Science and Standards, 1200 Montreal Road, Building M-12, Ottawa, Ontario K1A 0R6, Canada. 5. Department of Medicine, University of Perugia, Piazzale Gambuli, S.Andrea delle Fratte, 06132 Perugia, Italy. 6. Institute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Viet Nam. Electronic address: thaodo@ibt.ac.vn.
Abstract
OBJECTIVE: To investigate the antitumor effect of maesopsin 4-O-β-glucoside (TAT2) isolated from the leaves of Artocarpus tonkinensis (A. tonkinensis) A. Chev. ex Gagnep. METHODS: The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma (LLC) tumor-bearing mice. BALB/c mice had tumors induced by implantation with 2 × 10(6) LLC cells into the subcutaneous right posterior flank. Tumor-bearing mice were treated orally with a range of doses of TAT2 and a standard drug, doxorubicin. Animals were observed for tumor growth and mortality rate. Blood was collected to determine hematological and biochemical parameters. RESULTS: TAT2 was isolated from an ethanolic extract of A. tonkinensis leaves. Its structure was determined by MS and NMR spectroscopy, and identified as TAT2. The compound did not show acute toxicity at the highest dose tested (2000 mg/kg body weight). TAT2 exhibited antitumor activity by decreasing tumor growth, increasing the survival rate, and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight (P < 0.05). CONCLUSIONS: These results indicate that TAT2 possesses clear antitumor activity. Due to its bioavailability and low toxicity, and the fact that it could be isolated in a large scale from A. tonkinensis leaves, the compound shows promise as a potential anticancer drug.
OBJECTIVE: To investigate the antitumor effect of maesopsin 4-O-β-glucoside (TAT2) isolated from the leaves of Artocarpus tonkinensis (A. tonkinensis) A. Chev. ex Gagnep. METHODS: The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma (LLC) tumor-bearing mice. BALB/c mice had tumors induced by implantation with 2 × 10(6) LLC cells into the subcutaneous right posterior flank. Tumor-bearing mice were treated orally with a range of doses of TAT2 and a standard drug, doxorubicin. Animals were observed for tumor growth and mortality rate. Blood was collected to determine hematological and biochemical parameters. RESULTS: TAT2 was isolated from an ethanolic extract of A. tonkinensis leaves. Its structure was determined by MS and NMR spectroscopy, and identified as TAT2. The compound did not show acute toxicity at the highest dose tested (2000 mg/kg body weight). TAT2 exhibited antitumor activity by decreasing tumor growth, increasing the survival rate, and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight (P < 0.05). CONCLUSIONS: These results indicate that TAT2 possesses clear antitumor activity. Due to its bioavailability and low toxicity, and the fact that it could be isolated in a large scale from A. tonkinensis leaves, the compound shows promise as a potential anticancer drug.
Authors: Elena Orecchini; Giada Mondanelli; Ciriana Orabona; Claudia Volpi; Sabrina Adorisio; Mario Calvitti; Trinh Thi Thuy; Domenico V Delfino; Maria Laura Belladonna Journal: Front Pharmacol Date: 2021-01-22 Impact factor: 5.810