Turkmen Suha1, Mutlu Asli2, Sahin Aynur2, Karaca Yunus2, Mentese Ahmet3, Demir Selim3, Yulug Esin4, Tatli Ozgur2, Neziha Senem Ari4, Turedi Süleyman2. 1. Faculty of Medicine, Department of Emergency Medicine, Karadeniz Technical University, Trabzon, Turkey. Electronic address: drsuhaturkmen@hotmail.com. 2. Faculty of Medicine, Department of Emergency Medicine, Karadeniz Technical University, Trabzon, Turkey. 3. Faculty of Medicine, Department of Biochemistry, Karadeniz Technical University, Trabzon, Turkey. 4. Faculty of Medicine, Department of Histology, Karadeniz Technical University, Trabzon, Turkey.
Abstract
OBJECTIVES: This study was planned as a histopathologic evaluation of the effectiveness of ethyl pyruvate (EP) and N-acetylcysteine (NAC) in reducing electric burn-related organ damage in an experimental model. METHODS: Four groups of 7 female Wister rats were established. The first was a sham group, the second an electrical burn control group receiving 600 V, the third group received NAC therapy of 20 mg/kg 1 hour after 600 V electrical burn, and the fourth group received 50 mg/kg EP 1 hour after 600 V electrical burn. Heart, kidney, striated muscle, brain, and lung tissues obtained 24 hours postprocedurally were subjected to histopathologic examination, and injury scores were determined. The values determined were then subjected to statistical analysis. RESULTS: Electrical fire caused significant damage in heart, striated muscle, kidney, and brain tissues. A statistically significant decrease in injury scores in total striated muscle and heart tissue was observed in the 2 treatment groups administered NAC or EP compared to the control group (P= .001). Total kidney injury scores among the groups were significantly lower in the NAC and EP groups compared to the control group (P= .002 and P= .001, respectively). Brain injury examination revealed a significant decrease in injury scores with NAC and EP therapy, both antioxidant agents, in terms of neuron degeneration (P= .004 and P= .001, respectively). CONCLUSIONS: Electrical burn was observed to cause injury in heart, striated muscle, kidney, and brain tissue. This injury was reduced by the administration of NAC and EP.
OBJECTIVES: This study was planned as a histopathologic evaluation of the effectiveness of ethyl pyruvate (EP) and N-acetylcysteine (NAC) in reducing electric burn-related organ damage in an experimental model. METHODS: Four groups of 7 female Wister rats were established. The first was a sham group, the second an electrical burn control group receiving 600 V, the third group received NAC therapy of 20 mg/kg 1 hour after 600 V electrical burn, and the fourth group received 50 mg/kg EP 1 hour after 600 V electrical burn. Heart, kidney, striated muscle, brain, and lung tissues obtained 24 hours postprocedurally were subjected to histopathologic examination, and injury scores were determined. The values determined were then subjected to statistical analysis. RESULTS: Electrical fire caused significant damage in heart, striated muscle, kidney, and brain tissues. A statistically significant decrease in injury scores in total striated muscle and heart tissue was observed in the 2 treatment groups administered NAC or EP compared to the control group (P= .001). Total kidney injury scores among the groups were significantly lower in the NAC and EP groups compared to the control group (P= .002 and P= .001, respectively). Brain injury examination revealed a significant decrease in injury scores with NAC and EP therapy, both antioxidant agents, in terms of neuron degeneration (P= .004 and P= .001, respectively). CONCLUSIONS: Electrical burn was observed to cause injury in heart, striated muscle, kidney, and brain tissue. This injury was reduced by the administration of NAC and EP.
Authors: Christine Rossmann; Christoph Nusshold; Margret Paar; Gerhard Ledinski; Erwin Tafeit; Martin Koestenberger; Eva Maria Bernhart; Wolfgang Sattler; Gerhard Cvirn; Seth Hallström Journal: PLoS One Date: 2018-01-25 Impact factor: 3.240