Antônio Sérgio Barcala Jorge1, João Marcus Oliveira Andrade2, Alanna Fernandes Paraíso2, Gislaine Candida Batista Jorge2, Christine Mendes Silveira1, Ludmilla Regina de Souza2, Erivelton Pereira Santos2, André Luiz Sena Guimaraes3, Sérgio Henrique Sousa Santos3, Alfredo Maurício Batista De-Paula4. 1. Nucleus of Epidemiological and Molecular Research Catrumano. Health Research Laboratory. Health Science Post-graduate Programme, Universidade Estadual de Montes Claros, 39401-001 Montes Claros, MG, Brazil; Department of Physiopathology, Universidade Estadual de Montes Claros, 39401-001 Montes Claros, MG, Brazil. 2. Nucleus of Epidemiological and Molecular Research Catrumano. Health Research Laboratory. Health Science Post-graduate Programme, Universidade Estadual de Montes Claros, 39401-001 Montes Claros, MG, Brazil. 3. Nucleus of Epidemiological and Molecular Research Catrumano. Health Research Laboratory. Health Science Post-graduate Programme, Universidade Estadual de Montes Claros, 39401-001 Montes Claros, MG, Brazil; Department of Food Engineering, Universidade Federal de Minas Gerais, 39404-547 Montes Claros, MG, Brazil. 4. Nucleus of Epidemiological and Molecular Research Catrumano. Health Research Laboratory. Health Science Post-graduate Programme, Universidade Estadual de Montes Claros, 39401-001 Montes Claros, MG, Brazil; Department of Dentistry, Universidade Estadual de Montes Claros, 39401-001 Montes Claros, MG, Brazil. Electronic address: ambpatologi@gmail.com.
Abstract
OBJECTIVES: To analyze the mRNA expression of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in the liver and white adipose tissue samples of individuals with class III obesity (body mass index ≥40.0kg/m2) with non-alcoholic fatty liver disease (NAFLD). METHODS: This cross-sectional study included patients with class III obesity exhibiting early or late morphological presentation of NAFLD (non-alcoholic hepatic steatosis [NAFL], n=8 and non-alcoholic steatohepatitis [NASH], n=13, respectively). All patients underwent bariatric surgery and peripheral blood, liver, and visceral white adipose tissue (WAT) samples were collected. Socio-demographic, anthropometric, clinical, plasma biochemical, and nutritional characteristics of each study subject were assessed and compared between patients presenting with NAFL and NASH. IL-6 and TNF-α mRNA expression in the liver and WAT samples were measured by using quantitative real time-polymerase chain reaction (qRT-PCR). RESULTS: Individuals with class III obesity and NASH showed higher body mass index (BMI) and higher IL-6 and TNF-α mRNA expression in the WAT compared to that of patients with NAFL (p=0.01, for all associations). CONCLUSIONS: Individuals with class III obesity with higher morphological severity of NAFLD exhibited higher BMI and higher IL-6 and TNF-α expression in the WAT. Future prospective studies are warranted to determine how BMI, IL-6, and TNF-α affect the progression of NAFLD in individuals with class III obesity.
OBJECTIVES: To analyze the mRNA expression of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in the liver and white adipose tissue samples of individuals with class III obesity (body mass index ≥40.0kg/m2) with non-alcoholic fatty liver disease (NAFLD). METHODS: This cross-sectional study included patients with class III obesity exhibiting early or late morphological presentation of NAFLD (non-alcoholic hepatic steatosis [NAFL], n=8 and non-alcoholic steatohepatitis [NASH], n=13, respectively). All patients underwent bariatric surgery and peripheral blood, liver, and visceral white adipose tissue (WAT) samples were collected. Socio-demographic, anthropometric, clinical, plasma biochemical, and nutritional characteristics of each study subject were assessed and compared between patients presenting with NAFL and NASH. IL-6 and TNF-α mRNA expression in the liver and WAT samples were measured by using quantitative real time-polymerase chain reaction (qRT-PCR). RESULTS: Individuals with class III obesity and NASH showed higher body mass index (BMI) and higher IL-6 and TNF-α mRNA expression in the WAT compared to that of patients with NAFL (p=0.01, for all associations). CONCLUSIONS: Individuals with class III obesity with higher morphological severity of NAFLD exhibited higher BMI and higher IL-6 and TNF-α expression in the WAT. Future prospective studies are warranted to determine how BMI, IL-6, and TNF-α affect the progression of NAFLD in individuals with class III obesity.
Authors: Frank T Spradley; Jillian A Smith; Barbara T Alexander; Christopher D Anderson Journal: Am J Physiol Endocrinol Metab Date: 2018-03-06 Impact factor: 4.310
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