Tzu-Lun Huang1, Yao-Tseng Wen2, Chung-Hsing Chang3, Shu-Wen Chang4, Kung-Hung Lin5, Rong-Kung Tsai6. 1. Department of Ophthalmology, Far Eastern Memorial Hospital, Banciao District, New Taipei City, Taiwan 2Department of Electrical Engineering, Yuan-Ze University, Chung-Li, Taoyuan, Taiwan 3Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan. 2. Institute of Eye Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan. 3. Department of Dermatology, Kaohsiung Medical University Chun-Ho Memorial Hospital, Kaohsiung, Taiwan 6Department of Dermatology, Kaohsiung Medical University, Kaohsiung, Taiwan. 4. Department of Ophthalmology, Far Eastern Memorial Hospital, Banciao District, New Taipei City, Taiwan. 5. Department of Neurology, Taiwan Adventist Hospital, Taipei, Taiwan. 6. Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan 4Institute of Eye Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan.
Abstract
PURPOSE: To investigate effects of intravitreal injections of triamcinolone acetonide (IVI-TA) at different times in a rodent model of nonarteritic anterior ischemic optic neuropathy (rAION). METHODS: After inducing ischemic optic neuropathy, the rats received either IVI-TA (0.32 mg/2 μL) at 1 day (1d-TA), 1 week (7d-TA), 2 weeks (14d-TA), or PBS. The density of retinal ganglion cells (RGCs) was calculated using retrograde Fluorogold labeling. Electrophysiological visual function was assessed by flash visual evoked potentials (FVEPs). Apoptosis assays of the retinal sections and immunohistochemistry of ED1 staining of the optic nerves were performed. RESULTS: Four weeks postinfarct, the 1d- and 7d-TA groups had significantly rescued RGCs in the central (2160 ± 250 mm(2), P = 0.004; 2050 ± 660, P = 0.008, respectively) and midperipheral retinas (1240 ± 130; 1250 ± 220, respectively, both P = 0.004) compared with those of the PBS-treated rats. Flash visual evoked potentials demonstrated improvements in P1 amplitude (μV) in the 1d- and 7d-TA groups (both P < 0.05). Assays of TUNEL showed a decrease in the number of apoptotic cells in the RGC layers of 1d- and 7d-TA-treated retinas compared with the PBS-treated group (both P = 0.004). Cells ED1-positive were significantly decreased in the optic nerve (ON) of the 1d- and 7d-TA groups compared with the PBS group (P = 0.004 and 0.02, respectively). CONCLUSIONS: Within 1 week postinfarct of rAION, IVI-TA had neuroprotective effects on RGC survival with an increase in the electrophysiological amplitude of VEPs and a decrease in microglial infiltration in the ONs.
PURPOSE: To investigate effects of intravitreal injections of triamcinolone acetonide (IVI-TA) at different times in a rodent model of nonarteritic anterior ischemic optic neuropathy (rAION). METHODS: After inducing ischemic optic neuropathy, the rats received either IVI-TA (0.32 mg/2 μL) at 1 day (1d-TA), 1 week (7d-TA), 2 weeks (14d-TA), or PBS. The density of retinal ganglion cells (RGCs) was calculated using retrograde Fluorogold labeling. Electrophysiological visual function was assessed by flash visual evoked potentials (FVEPs). Apoptosis assays of the retinal sections and immunohistochemistry of ED1 staining of the optic nerves were performed. RESULTS: Four weeks postinfarct, the 1d- and 7d-TA groups had significantly rescued RGCs in the central (2160 ± 250 mm(2), P = 0.004; 2050 ± 660, P = 0.008, respectively) and midperipheral retinas (1240 ± 130; 1250 ± 220, respectively, both P = 0.004) compared with those of the PBS-treated rats. Flash visual evoked potentials demonstrated improvements in P1 amplitude (μV) in the 1d- and 7d-TA groups (both P < 0.05). Assays of TUNEL showed a decrease in the number of apoptotic cells in the RGC layers of 1d- and 7d-TA-treated retinas compared with the PBS-treated group (both P = 0.004). Cells ED1-positive were significantly decreased in the optic nerve (ON) of the 1d- and 7d-TA groups compared with the PBS group (P = 0.004 and 0.02, respectively). CONCLUSIONS: Within 1 week postinfarct of rAION, IVI-TA had neuroprotective effects on RGC survival with an increase in the electrophysiological amplitude of VEPs and a decrease in microglial infiltration in the ONs.
Authors: Yan Guo; Zara Mehrabian; Mary A Johnson; David S Albers; Cadmus C Rich; Rudolf A Baumgartner; Steven L Bernstein Journal: Transl Vis Sci Technol Date: 2019-12-20 Impact factor: 3.283
Authors: Yan Guo; Zara Mehrabian; Mary A Johnson; Neil R Miller; Amanda D Henderson; John Hamlyn; Steven L Bernstein Journal: PLoS One Date: 2021-03-25 Impact factor: 3.240