Lívia Santos1,2, Marta Silva1,2, Ana I Gonçalves1,2, Tamagno Pesqueira1,2, Márcia T Rodrigues1,2, Manuela E Gomes1,2. 1. 3B's Research Group, Biomaterials, Biodegradables & Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Portugal. 2. ICVS/3B's, PT Government Associate Laboratory, Portugal.
Abstract
AIM: To expand our understanding on the effect of magnetically actuated biomaterials in stem cells, inflammation and fibrous tissue growth. MATERIALS & METHODS: Magnetic biomaterials were obtained by doping iron oxide particles into starch poly-ϵ-caprolactone (SPCL) to create two formulations, magSPCL-1.8 and 3.6. Stem cell behavior was assessed in vitro and the inflammatory response, subcutaneously in Wistar rats. RESULTS: Metabolic activity and proliferation increased significantly overtime in SPCL and magSPCL-1.8. Electromagnetic fields attenuated the presence of mast cells and macrophages in tissues surrounding SPCL and magSPCL-1.8, between weeks 1 and 9. Macrophage reduction was more pronounced for magSPCL-1.8, which could explain why this material prevented growth of fibrous tissue overtime. CONCLUSION: Magnetically actuated biomaterials have potential to modulate inflammation and the growth of fibrous tissue.
AIM: To expand our understanding on the effect of magnetically actuated biomaterials in stem cells, inflammation and fibrous tissue growth. MATERIALS & METHODS: Magnetic biomaterials were obtained by doping iron oxide particles into starch poly-ϵ-caprolactone (SPCL) to create two formulations, magSPCL-1.8 and 3.6. Stem cell behavior was assessed in vitro and the inflammatory response, subcutaneously in Wistar rats. RESULTS: Metabolic activity and proliferation increased significantly overtime in SPCL and magSPCL-1.8. Electromagnetic fields attenuated the presence of mast cells and macrophages in tissues surrounding SPCL and magSPCL-1.8, between weeks 1 and 9. Macrophage reduction was more pronounced for magSPCL-1.8, which could explain why this material prevented growth of fibrous tissue overtime. CONCLUSION: Magnetically actuated biomaterials have potential to modulate inflammation and the growth of fibrous tissue.
Entities:
Keywords:
electromagnetic fields; magnetic responsive biomaterials; regenerative medicine