Hai Fang Chen1, You Hua Chen2, Chun Hua Liu2, Lu Wang2, Xi Chen2, Bo Yang Yu3, Jin Qi3. 1. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Road, Nanjing, 210009 China ; Key Laboratory of Modern Preparation of TCM of Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004 China. 2. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Road, Nanjing, 210009 China. 3. Department of Complex Prescription of Traditional Chinese Medicine, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 639 Longmian Road, Nanjing, 211198 China.
Abstract
BACKGROUND: The processed roots of Polygonum multiflorum Thunb. (Heshouwu; processed HSW) are commonly used in anti-aging medicine. Few reports have combined chemical profiles with bioactivity to evaluate the quality of the processed HSW. This study aims to integrate chemometric fingerprints of antioxidant activities and high-performance liquid chromatography-diode array detection-chemiluminescence (HPLC-DAD-CL) to assess the quality of processed HSW. METHODS: An online HPLC-DAD-CL based on the three reactive oxygen species (ROS), superoxide anion, hydrogen peroxide, and peroxynitriteanion, was developed to screen the potential anti-aging constituents for a comprehensive quality evaluation of processed HSW. Additionally, antioxidant-activity-integrated fingerprints were constructed and hierarchical cluster analysis and principal component analysis were used to evaluate the variations among 14 batches of processed HSW samples purchased from drug stores in different habitats. RESULTS: Fourteen batches of processed HSW samples were highly similar and classified into two clusters using hierarchical cluster analysis. Twelve active compounds exhibited antioxidant activity on the ROS with different degrees of sensitivity that constituted specific fingerprints. Among them, protocatechuic acid, catechin, trans-2,3,5,4'-tetrahydroxy-stilbene-2-O-β-d-glucoside, 2,3,5, 4'-tetrahydroxy-stilbene-2-O-β-d-(2''-galloyl)-glucoside, torachrysone-8-O-glucoside, and emodin-8-O-β-d-glucoside exerted relatively large influences on the differences between processed HSW samples. CONCLUSION: Our study established the antioxidative activity-integrated fingerprint for processed HSW and achieved a screening of the potential anti-aging constituents using the online HPLC-DAD-CL method with H2O2, O2 (•-), and ONOO(-)scavenging experiments.
BACKGROUND: The processed roots of Polygonum multiflorum Thunb. (Heshouwu; processed HSW) are commonly used in anti-aging medicine. Few reports have combined chemical profiles with bioactivity to evaluate the quality of the processed HSW. This study aims to integrate chemometric fingerprints of antioxidant activities and high-performance liquid chromatography-diode array detection-chemiluminescence (HPLC-DAD-CL) to assess the quality of processed HSW. METHODS: An online HPLC-DAD-CL based on the three reactive oxygen species (ROS), superoxide anion, hydrogen peroxide, and peroxynitriteanion, was developed to screen the potential anti-aging constituents for a comprehensive quality evaluation of processed HSW. Additionally, antioxidant-activity-integrated fingerprints were constructed and hierarchical cluster analysis and principal component analysis were used to evaluate the variations among 14 batches of processed HSW samples purchased from drug stores in different habitats. RESULTS: Fourteen batches of processed HSW samples were highly similar and classified into two clusters using hierarchical cluster analysis. Twelve active compounds exhibited antioxidant activity on the ROS with different degrees of sensitivity that constituted specific fingerprints. Among them, protocatechuic acid, catechin, trans-2,3,5,4'-tetrahydroxy-stilbene-2-O-β-d-glucoside, 2,3,5, 4'-tetrahydroxy-stilbene-2-O-β-d-(2''-galloyl)-glucoside, torachrysone-8-O-glucoside, and emodin-8-O-β-d-glucoside exerted relatively large influences on the differences between processed HSW samples. CONCLUSION: Our study established the antioxidative activity-integrated fingerprint for processed HSW and achieved a screening of the potential anti-aging constituents using the online HPLC-DAD-CL method with H2O2, O2 (•-), and ONOO(-)scavenging experiments.
Authors: Hyun Ki Kim; Yeon Hee Choi; Jae Seok Choi; Sang Un Choi; Young Sup Kim; Kang Ro Lee; Young-Kyoon Kim; Shi Yong Ryu Journal: Arch Pharm Res Date: 2008-10-29 Impact factor: 4.946