Mohamed H Abdelraheem1, Musab M A Albsheer2, Hiba S Mohamed2, Mutaz Amin3, Muzamil Mahdi Abdel Hamid4. 1. Institute of Endemic Diseases, University of Khartoum, Sudan National Centre for Research Commission for Biotechnology and Genetic Engineering. 2. Institute of Endemic Diseases, University of Khartoum, Sudan. 3. Institute of Endemic Diseases, University of Khartoum, Sudan Department of Biochemistry, Faculty of Medicine, University of Khartoum, Sudan. 4. Institute of Endemic Diseases, University of Khartoum, Sudan Mahdi@iend.org.
Abstract
BACKGROUND: Due to the recently observed rise in Plasmodium vivax incidence in Sudan and reported transmission in Duffy-negative individuals; we aimed to assess the possibility of P. vivax transmission in Duffy-negative individuals in Gezira state, central Sudan. METHOD: A total of 126 suspected malaria patients were diagnosed with P. vivax infection using microscopy, RDT and PCR. PCR-RFLP was used to genotype participants Duffy status. RESULTS: Forty eight (38%) were positive for P. vivax infection by PCR. Four patients (8.3%) were homozygous Duffy-negative. CONCLUSION: These results confirm that P. vivax can infect Duffy-negative individuals, suggesting alternative mechanisms to bind and invade erythrocytes.
BACKGROUND: Due to the recently observed rise in Plasmodium vivax incidence in Sudan and reported transmission in Duffy-negative individuals; we aimed to assess the possibility of P. vivax transmission in Duffy-negative individuals in Gezira state, central Sudan. METHOD: A total of 126 suspected malariapatients were diagnosed with P. vivaxinfection using microscopy, RDT and PCR. PCR-RFLP was used to genotype participants Duffy status. RESULTS: Forty eight (38%) were positive for P. vivaxinfection by PCR. Four patients (8.3%) were homozygous Duffy-negative. CONCLUSION: These results confirm that P. vivax can infect Duffy-negative individuals, suggesting alternative mechanisms to bind and invade erythrocytes.
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