HongTao Wang1, Seok-Mo Heo2, Heung Yong Jin3, Eui Yul Choi1, Sang Wook Oh4. 1. BodiTechMed Inc., Chuncheon, South Korea. 2. Department of Periodontology, School of Dentistry, Chonbuk National University, Jeonju, South Korea. 3. Division of Endocrinology and Metabolism, Chonbuk National University of Medical School, Jeonju, South Korea. 4. Department of Biology Education, Institute of Fusion Science, Chonbuk National University, Jeonju, South Korea. sangwoh@chonbuk.ac.kr.
Abstract
BACKGROUND: Recently, the human common salivary protein 1 (CSP1) was identified as an ortholog of the Demilune cell and parotid protein of mouse. However, its function remains to be determined. Here, we show that the serum CSP1 concentration of diabetes mellitus (DM) patients is much higher than that of healthy controls. METHODS: Recombinant human CSP1 was expressed as a Glutathione-S-transferase (GST)-tagged protein, and the purified fusion protein was used as an immunogen to generate monoclonal antibody (mAb) to CSP1. The produced mAb was tested as a probe in Western blotting of human saliva and in immunohistochemistry of various human tissues. The serum CSP1 levels of 31 DM patients and 38 normal adults were quantified by a house-fabricated CSP1 sandwich enzyme-linked immunosorbent assay (ELISA) system. RESULTS: Immunoblot analysis by mAb-hCSP1#4 showed that CSP1 in human saliva exists in a 27 kDa glycosylated form. Among the various human tissues tested, the salivary gland was the only tissue stained with mAb-hCSP1#4 by immunohistochemistry. Quantification of serum CSP1 concentration by CSP1 ELISA showed that the median values (25th-75th percentile) of DM patients and healthy adults were 22.2 (15.8-28.2) and 3.2 (0-11.4), respectively. Student's t-test results indicated that there was a statistically significant difference between the two groups (P < 0.01). CONCLUSION: The significant difference between the CSP1 levels of the two groups indicated that CSP1 would be a potential biomarker for detection or screening of DM patients.
BACKGROUND: Recently, the humancommon salivary protein 1 (CSP1) was identified as an ortholog of the Demilune cell and parotid protein of mouse. However, its function remains to be determined. Here, we show that the serum CSP1 concentration of diabetes mellitus (DM) patients is much higher than that of healthy controls. METHODS: Recombinant humanCSP1 was expressed as a Glutathione-S-transferase (GST)-tagged protein, and the purified fusion protein was used as an immunogen to generate monoclonal antibody (mAb) to CSP1. The produced mAb was tested as a probe in Western blotting of human saliva and in immunohistochemistry of various human tissues. The serum CSP1 levels of 31 DMpatients and 38 normal adults were quantified by a house-fabricated CSP1 sandwich enzyme-linked immunosorbent assay (ELISA) system. RESULTS: Immunoblot analysis by mAb-hCSP1#4 showed that CSP1 in human saliva exists in a 27 kDa glycosylated form. Among the various human tissues tested, the salivary gland was the only tissue stained with mAb-hCSP1#4 by immunohistochemistry. Quantification of serum CSP1 concentration by CSP1 ELISA showed that the median values (25th-75th percentile) of DMpatients and healthy adults were 22.2 (15.8-28.2) and 3.2 (0-11.4), respectively. Student's t-test results indicated that there was a statistically significant difference between the two groups (P < 0.01). CONCLUSION: The significant difference between the CSP1 levels of the two groups indicated that CSP1 would be a potential biomarker for detection or screening of DMpatients.
Authors: Thomas V O Hansen; Mette K Simonsen; Finn C Nielsen; Yrsa Andersen Hundrup Journal: Cancer Epidemiol Biomarkers Prev Date: 2007-10 Impact factor: 4.254
Authors: John J Mullins; Linda J Mullins; Donald R Dunbar; William J Brammar; Kenneth W Gross; Steven D Morley Journal: Physiol Genomics Date: 2006-09-05 Impact factor: 3.107
Authors: Kiran S Ambatipudi; Fred K Hagen; Claire M Delahunty; Xuemei Han; Rubina Shafi; Jennifer Hryhorenko; Stacy Gregoire; Robert E Marquis; James E Melvin; Hyun Koo; John R Yates Journal: J Proteome Res Date: 2010-10-22 Impact factor: 4.466
Authors: Sun A Kim; Yangsoon Lee; Dawoon E Jung; Kyung Hwa Park; Jeong Youp Park; Jingu Gang; Sun Bok Jeon; Eui Chul Park; Young-Gun Kim; Bogman Lee; Qing Liu; Wen Zeng; Subramanyam Yeramilli; Soojin Lee; Sang Seok Koh; Si Young Song Journal: Cancer Sci Date: 2009-03-02 Impact factor: 6.716