Literature DB >> 27074833

A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity.

Tianming Yang1, Adeline Cheong, Xiangrui Mai, Shui Zou, Esther C Y Woon.   

Abstract

We describe a novel methylation-sensitive nucleic acid (RNA) probe which switches conformation according to its methylation status. When combined with a differential scanning fluorimetry technique, it enables highly sensitive and selective detection of demethylase activity at a single methylated-base level. The approach is highly versatile and may be adapted to a broad range of RNA demethylases.

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Year:  2016        PMID： 27074833     DOI： 10.1039/c6cc01045h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  8 in total

1.  Fluorescence Probes for ALKBH2 Allow the Measurement of DNA Alkylation Repair and Drug Resistance Responses.

Authors:  David L Wilson; Andrew A Beharry; Avinash Srivastava; Timothy R O'Connor; Eric T Kool
Journal:  Angew Chem Int Ed Engl       Date:  2018-09-03       Impact factor: 15.336

2.  N 6-methyladenosine binding induces a metal-centered rearrangement that activates the human RNA demethylase Alkbh5.

Authors:  Jeffrey A Purslow; Trang T Nguyen; Balabhadra Khatiwada; Aayushi Singh; Vincenzo Venditti
Journal:  Sci Adv       Date:  2021-08-18       Impact factor: 14.136

Review 3.  m1A RNA Modification in Gene Expression Regulation.

Authors:  Hao Jin; Chunxiao Huo; Tianhua Zhou; Shanshan Xie
Journal:  Genes (Basel)       Date:  2022-05-19       Impact factor: 4.141

4.  Active Site Breathing of Human Alkbh5 Revealed by Solution NMR and Accelerated Molecular Dynamics.

Authors:  Jeffrey A Purslow; Trang T Nguyen; Timothy K Egner; Rochelle R Dotas; Balabhadra Khatiwada; Vincenzo Venditti
Journal:  Biophys J       Date:  2018-10-11       Impact factor: 4.033

5.  N(6)-Methyladenosine: a conformational marker that regulates the substrate specificity of human demethylases FTO and ALKBH5.

Authors:  Shui Zou; Joel D W Toh; Kendra H Q Wong; Yong-Gui Gao; Wanjin Hong; Esther C Y Woon
Journal:  Sci Rep       Date:  2016-05-09       Impact factor: 4.379

6.  m(1)A and m(1)G disrupt A-RNA structure through the intrinsic instability of Hoogsteen base pairs.

Authors:  Huiqing Zhou; Isaac J Kimsey; Evgenia N Nikolova; Bharathwaj Sathyamoorthy; Gianmarc Grazioli; James McSally; Tianyu Bai; Christoph H Wunderlich; Christoph Kreutz; Ioan Andricioaei; Hashim M Al-Hashimi
Journal:  Nat Struct Mol Biol       Date:  2016-08-01       Impact factor: 15.369

7.  A general strategy exploiting m5C duplex-remodelling effect for selective detection of RNA and DNA m5C methyltransferase activity in cells.

Authors:  Tianming Yang; Joanne J A Low; Esther C Y Woon
Journal:  Nucleic Acids Res       Date:  2020-01-10       Impact factor: 16.971

8.  A fluorescent methylation-switchable probe for highly sensitive analysis of FTO N 6-methyladenosine demethylase activity in cells.

Authors:  Adeline Cheong; Joanne J A Low; Andrea Lim; Paul M Yen; Esther C Y Woon
Journal:  Chem Sci       Date:  2018-08-07       Impact factor: 9.825
  8 in total

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