Wenbo Wang1, Jing He2, Bei Feng3, Zhiyong Zhang1,4, Wenjie Zhang1,4, Guangdong Zhou1,4, Yilin Cao1,4, Wei Fu3, Wei Liu1,4. 1. Department of Plastic & Reconstructive Surgery, Shanghai Tissue Engineering Key Laboratory, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, PR China. 2. Department of Anatomy & Neurobiology, Tongji University School of Medicine, Shanghai, PR China. 3. Department of Pediatric Cardiothoracic Surgery, Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao University, Shanghai, PR China. 4. National Tissue Engineering Center of China, Shanghai, PR China.
Abstract
AIM: To explore the effect of aligned nanofibers on inducing tenogenic phenotype of human dermal fibroblasts (hDFs) in vitro and on inducing de novo tendon regeneration in vivo. MATERIALS & METHODS: Random and aligned nanofibers were electrospun, seeded with hDFs and cultured in vitro, and in vivo implanted without cell seeding to bridge segmental defect of rat Achilles tendon. RESULTS: In vitro, the well-aligned nanofibers could elongate hDFs, induce a tenogenic phenotype and form better organized neotendon respectively compared with random nanofibers. In vivo, the bridged nanofibers of aligned group could better recruit host cells and regenerate Achilles tendon de novo with enhanced tenogenic gene expression. CONCLUSION: Aligned nanofibers could induce tenogenic phenotype in vitro and regenerate tendon in vivo.
AIM: To explore the effect of aligned nanofibers on inducing tenogenic phenotype of human dermal fibroblasts (hDFs) in vitro and on inducing de novo tendon regeneration in vivo. MATERIALS & METHODS: Random and aligned nanofibers were electrospun, seeded with hDFs and cultured in vitro, and in vivo implanted without cell seeding to bridge segmental defect of rat Achilles tendon. RESULTS: In vitro, the well-aligned nanofibers could elongate hDFs, induce a tenogenic phenotype and form better organized neotendon respectively compared with random nanofibers. In vivo, the bridged nanofibers of aligned group could better recruit host cells and regenerate Achilles tendon de novo with enhanced tenogenic gene expression. CONCLUSION: Aligned nanofibers could induce tenogenic phenotype in vitro and regenerate tendon in vivo.
Entities:
Keywords:
human dermal fibroblasts; nanofibers; tendon regeneration; tenogenic transdifferentiation
Authors: Marcello de Alencar Silva; Angela Faustino Jozala; Maria Acelina Martins de Carvalho; Yulla Klinger de Carvalho Leite; Camila Ernanda Sousa de Carvalho; Matheus Levi Tajra Feitosa; Michel Muálem de Moraes Alves; Fernando Aécio de Amorim Carvalho; Bartolomeu Cruz Viana Neto; Maria Angélica Miglino Journal: PeerJ Date: 2018-04-30 Impact factor: 2.984
Authors: Valentina Russo; Mohammad El Khatib; Lisa di Marcantonio; Massimo Ancora; Ralf Wyrwa; Annunziata Mauro; Torsten Walter; Jürgen Weisser; Maria Rita Citeroni; Francesco Lazzaro; Marta Di Federico; Paolo Berardinelli; Cesare Cammà; Matthias Schnabelrauch; Barbara Barboni Journal: Cells Date: 2020-01-27 Impact factor: 6.600
Authors: Mohammad El Khatib; Annunziata Mauro; Miriam Di Mattia; Ralf Wyrwa; Martina Schweder; Massimo Ancora; Francesco Lazzaro; Paolo Berardinelli; Luca Valbonetti; Oriana Di Giacinto; Andrea Polci; Cesare Cammà; Matthias Schnabelrauch; Barbara Barboni; Valentina Russo Journal: Cells Date: 2020-05-13 Impact factor: 6.600