BACKGROUND: The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of neonatal infections in 5 population-based sites in Bangladesh, India and Pakistan. METHODS: The main laboratory challenges in ANISA were selection and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 0-59 days and (4) caregivers' concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple available options, all with limited field validation, for use in determining infection in young infants. CONCLUSIONS: This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.
BACKGROUND: The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of neonatal infections in 5 population-based sites in Bangladesh, India and Pakistan. METHODS: The main laboratory challenges in ANISA were selection and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 0-59 days and (4) caregivers' concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple available options, all with limited field validation, for use in determining infection in young infants. CONCLUSIONS: This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.
Authors: Melissa L Arvay; Nong Shang; Shamim A Qazi; Gary L Darmstadt; Mohammad Shahidul Islam; Daniel E Roth; Anran Liu; Nicholas E Connor; Belal Hossain; Qazi Sadeq-Ur Rahman; Shams El Arifeen; Luke C Mullany; Anita K M Zaidi; Zulfiqar A Bhutta; Sajid B Soofi; Yasir Shafiq; Abdullah H Baqui; Dipak K Mitra; Pinaki Panigrahi; Kalpana Panigrahi; Anuradha Bose; Rita Isaac; Daniel Westreich; Steven R Meshnick; Samir K Saha; Stephanie J Schrag Journal: Lancet Glob Health Date: 2022-09 Impact factor: 38.927
Authors: Maria Deloria Knoll; Wei Fu; Qiyuan Shi; Christine Prosperi; Zhenke Wu; Laura L Hammitt; Daniel R Feikin; Henry C Baggett; Stephen R C Howie; J Anthony G Scott; David R Murdoch; Shabir A Madhi; Donald M Thea; W Abdullah Brooks; Karen L Kotloff; Mengying Li; Daniel E Park; Wenyi Lin; Orin S Levine; Katherine L O'Brien; Scott L Zeger Journal: Clin Infect Dis Date: 2017-06-15 Impact factor: 9.079
Authors: Samir K Saha; Stephanie J Schrag; Shams El Arifeen; Luke C Mullany; Mohammad Shahidul Islam; Nong Shang; Shamim A Qazi; Anita K M Zaidi; Zulfiqar A Bhutta; Anuradha Bose; Pinaki Panigrahi; Sajid B Soofi; Nicholas E Connor; Dipak K Mitra; Rita Isaac; Jonas M Winchell; Melissa L Arvay; Maksuda Islam; Yasir Shafiq; Imran Nisar; Benazir Baloch; Furqan Kabir; Murtaza Ali; Maureen H Diaz; Radhanath Satpathy; Pritish Nanda; Bijaya K Padhi; Sailajanandan Parida; Aneeta Hotwani; M Hasanuzzaman; Sheraz Ahmed; Mohammad Belal Hossain; Shabina Ariff; Imran Ahmed; Syed Mamun Ibne Moin; Arif Mahmud; Jessica L Waller; Iftekhar Rafiqullah; Mohammad A Quaiyum; Nazma Begum; Veeraraghavan Balaji; Jasmin Halen; A S M Nawshad Uddin Ahmed; Martin W Weber; Davidson H Hamer; Patricia L Hibberd; Qazi Sadeq-Ur Rahman; Venkat Raghava Mogan; Tanvir Hossain; Lesley McGee; Shalini Anandan; Anran Liu; Kalpana Panigrahi; Asha Mary Abraham; Abdullah H Baqui Journal: Lancet Date: 2018-07-06 Impact factor: 79.321