Defu Chen1, Jie Ren2, Ying Wang2, Buhong Li3, Ying Gu4. 1. School of Information and Electronics, Beijing Institute of Technology, Beijing 100081, China. 2. Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China. 3. Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China. 4. School of Information and Electronics, Beijing Institute of Technology, Beijing 100081, China; Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China. Electronic address: guyinglaser301@163.com.
Abstract
OBJECTIVE: The objective of this study was to monitor blood perfusion dynamics of port wine stains (PWS) during vascular targeted photodynamic therapy (V-PDT) with laser Doppler imaging (LDI). METHODS: The PWS lesions of 30 facial PWS patients received V-PDT, while the normal skins on the forearm of 5 healthy subjects were treated as light-only controls for comparison. Furthermore, two different PWS lesions in the same individual from each of 3 PWS patients successively received laser irradiation only and V-PDT, respectively. LDI was used to monitor intraoperative blood perfusion dynamics. RESULTS: During V-PDT, the blood perfusion (278±96 PU) in PWS lesions for 31 of 33 PWS patients significantly increased after the initiation of V-PDT treatment, then reached a peak (638±105 PU) within 10min, followed by a slow decrease to a relatively lower level (515±100 PU). Furthermore, the time for reaching peak and the subsequent magnitude of decrease in blood perfusion varied with different patients. For light-only controls, an initial perfusion peak at 3min followed by a nadir and a secondary increase were found not only in normal skin, but also in PWS lesions. CONCLUSION: The preliminary results showed that the LDI permits non-invasive monitoring blood perfusion changes of PWS lesions during V-PDT. There was a clear trend in blood perfusion responses during V-PDT and laser irradiation. The blood perfusion changes during treatment were due to V-PDT effects as well as local temperature increase induced by laser irradiation.
OBJECTIVE: The objective of this study was to monitor blood perfusion dynamics of port wine stains (PWS) during vascular targeted photodynamic therapy (V-PDT) with laser Doppler imaging (LDI). METHODS: The PWS lesions of 30 facial PWSpatients received V-PDT, while the normal skins on the forearm of 5 healthy subjects were treated as light-only controls for comparison. Furthermore, two different PWS lesions in the same individual from each of 3 PWSpatients successively received laser irradiation only and V-PDT, respectively. LDI was used to monitor intraoperative blood perfusion dynamics. RESULTS: During V-PDT, the blood perfusion (278±96 PU) in PWS lesions for 31 of 33 PWSpatients significantly increased after the initiation of V-PDT treatment, then reached a peak (638±105 PU) within 10min, followed by a slow decrease to a relatively lower level (515±100 PU). Furthermore, the time for reaching peak and the subsequent magnitude of decrease in blood perfusion varied with different patients. For light-only controls, an initial perfusion peak at 3min followed by a nadir and a secondary increase were found not only in normal skin, but also in PWS lesions. CONCLUSION: The preliminary results showed that the LDI permits non-invasive monitoring blood perfusion changes of PWS lesions during V-PDT. There was a clear trend in blood perfusion responses during V-PDT and laser irradiation. The blood perfusion changes during treatment were due to V-PDT effects as well as local temperature increase induced by laser irradiation.
Authors: M Ingmar van Raath; Sandeep Chohan; Albert Wolkerstorfer; Chantal M A M van der Horst; Jacqueline Limpens; Xuan Huang; Baoyue Ding; Gert Storm; René R W J van der Hulst; Michal Heger Journal: PLoS One Date: 2020-07-02 Impact factor: 3.240