Geng Chen1,2, Shou-Ying Li2, Hamid Tayyab Malik2, Yu-Guang Ma3, Hong Xu4, Lian-Kun Sun5. 1. Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China. 2. Department of Gastroenterology, The First Hospital of Jilin University, No. 71 XinMin Street, Changchun, 130021, China. 3. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, 130021, China. 4. Department of Gastroenterology, The First Hospital of Jilin University, No. 71 XinMin Street, Changchun, 130021, China. chxuhong@163.com. 5. Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China. gengchen1981@163.com.
Abstract
OBJECTIVES: To investigate the biocompatibility of human gastric carcinoma cells (SGC-7901) with organic two-photon nanoparticles (NPs). RESULTS: Different concentrations of NPs were incubated with SGC-7901 cells for different times. The levels of cell apoptosis, reactive oxygen species (ROS), intracellular calcium, and mitochondrial membrane potential (MMP) were measured by staining the SGC-7901 cells with Annexin V-FITC/PI, 2',7'-dichlorofluorescin diacetate, Fluo-3 AM, and Rhodamine 123, followed by the flow cytometry assay. NPs at <4 µg/ml, did not have any significant effect on apoptosis, necrosis, generation of ROS, increase of intracellular Ca(2+) concentration or decrease of MMP in SGC-7901 cells, but >4 µg/ml had a major effects on all the above mentioned parameters. CONCLUSION: 2,5,2',5'-Tetra(4-N,N-diphenylamine styryl) biphenyl NPs can be used at an appropriate concentration as a safe drug carrier or imaging marker and may serve as an effective tool for developing a photodynamic cancer therapy.
OBJECTIVES: To investigate the biocompatibility of humangastric carcinoma cells (SGC-7901) with organic two-photon nanoparticles (NPs). RESULTS: Different concentrations of NPs were incubated with SGC-7901 cells for different times. The levels of cell apoptosis, reactive oxygen species (ROS), intracellular calcium, and mitochondrial membrane potential (MMP) were measured by staining the SGC-7901 cells with Annexin V-FITC/PI, 2',7'-dichlorofluorescin diacetate, Fluo-3 AM, and Rhodamine 123, followed by the flow cytometry assay. NPs at <4 µg/ml, did not have any significant effect on apoptosis, necrosis, generation of ROS, increase of intracellular Ca(2+) concentration or decrease of MMP in SGC-7901 cells, but >4 µg/ml had a major effects on all the above mentioned parameters. CONCLUSION: 2,5,2',5'-Tetra(4-N,N-diphenylamine styryl) biphenyl NPs can be used at an appropriate concentration as a safe drug carrier or imaging marker and may serve as an effective tool for developing a photodynamic cancer therapy.
Entities:
Keywords:
Apoptosis; Human gastric carcinoma SGC-7901 cells; Intracellular Ca2+; Mitochondrial membrane potential; Nanoparticles; Organic two-photon nanoparticles; Reactive oxygen species