Michael Sand1,2, Falk G Bechara1, Daniel Sand3, Thilo Gambichler1, Stephan A Hahn4, Michael Bromba2, Eggert Stockfleth1, Schapoor Hessam1. 1. Dermatologic Surgery Unit, Department of Dermatology, Venereology & Allergology, Ruhr-University Bochum, 44791 Bochum, Germany. 2. Department of Plastic Surgery, St Josef Hospital, Catholic Clinics of the Ruhr Peninsula, 45257 Essen, Germany. 3. University of Michigan Kellogg Eye Center, Ann Arbor, MI 48105, USA. 4. Department of Internal Medicine, Knappschaftskrankenhaus University of Bochum, Zentrum für Klinische Forschung, Labor für Molekulare Gastroenterologische Onkologie, 44780 Bochum, Germany.
Abstract
BACKGROUND: Despite there being over 35,000 different long noncoding RNA (lncRNA) sequences described little is known regarding their molecular-pathological role in cutaneous squamous cell carcinoma (cSCC). MATERIALS & METHODS: In this pilot study, lncRNA and mRNA expression profiles were determined in cSCC and control (n = 6) by an Arraystar human lncRNA Microarray. Kyoto Encyclopedia of Genes and Genomes pathway enrichment and gene ontology analysis of mRNAs was performed. RESULTS: Analysis of differential expression revealed 1516 upregulated lncRNAs and 2586 downregulated lncRNAs in cSCC compared with controls. Data analysis identified known oncogenic lncRNAs, such as the HOX transcript antisense RNA HOTAIR, among the differentially expressed lncRNA sequences. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that focal adhesion, extracellular matrix and the oncogenic phosphatidylinositol 3'-kinase-Akt signaling pathway had the highest enrichment scores. CONCLUSION: This study provides the first evidence for differential expression of lncRNA in cSCC and serves as a template for further, larger functional in-depth analyses regarding cSCC molecular lncRNAs.
BACKGROUND: Despite there being over 35,000 different long noncoding RNA (lncRNA) sequences described little is known regarding their molecular-pathological role in cutaneous squamous cell carcinoma (cSCC). MATERIALS & METHODS: In this pilot study, lncRNA and mRNA expression profiles were determined in cSCC and control (n = 6) by an Arraystar human lncRNA Microarray. Kyoto Encyclopedia of Genes and Genomes pathway enrichment and gene ontology analysis of mRNAs was performed. RESULTS: Analysis of differential expression revealed 1516 upregulated lncRNAs and 2586 downregulated lncRNAs in cSCC compared with controls. Data analysis identified known oncogenic lncRNAs, such as the HOX transcript antisense RNA HOTAIR, among the differentially expressed lncRNA sequences. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that focal adhesion, extracellular matrix and the oncogenic phosphatidylinositol 3'-kinase-Akt signaling pathway had the highest enrichment scores. CONCLUSION: This study provides the first evidence for differential expression of lncRNA in cSCC and serves as a template for further, larger functional in-depth analyses regarding cSCC molecular lncRNAs.
Entities:
Keywords:
cutaneous squamous cell carcinoma; epithelial skin cancer; long noncoding RNAs; noncoding RNAs; nonmelanoma skin cancer
Authors: Kunal Das Mahapatra; Lorenzo Pasquali; Jonas Nørskov Søndergaard; Jan Lapins; István Balazs Nemeth; Eszter Baltás; Lajos Kemény; Bernhard Homey; Liviu-Ionut Moldovan; Jørgen Kjems; Claudia Kutter; Enikö Sonkoly; Lasse Sommer Kristensen; Andor Pivarcsi Journal: Sci Rep Date: 2020-02-27 Impact factor: 4.379