Literature DB >> 27065614

Diagnosis of Trypanosoma evansi in bovines by indirect ELISA.

S Sivajothi1, V C Rayulu1, P Malakondaiah1, D Sreenivasulu1.   

Abstract

The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in bovines. The optimum concentration of antigen, test sera and conjugate were determined as 5 µg per well, 1:10 and 1: 6,000 dilutions, respectively. Among 320 cattle and 382 buffaloes examined in different parts of Rayalaseema region of Andhra Pradesh for T. evansi infection, 36.12 and 31.87 percent were found positive by indirect ELISA, respectively.

Entities:  

Keywords:  Antibodies; Bovines; Diagnosis; Indirect ELISA; Trypanosoma evansi

Year:  2014        PMID： 27065614      PMCID： PMC4815840          DOI： 10.1007/s12639-014-0465-z

Source DB:  PubMed          Journal:  J Parasit Dis        ISSN： 0971-7196


  6 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Haematological and biochemical changes in experimental Trypanosoma evansi infection in rabbits.

Authors:  S Sivajothi; V C Rayulu; B Sudhakara Reddy
Journal:  J Parasit Dis       Date:  2013-06-19

3.  Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.

Authors:  S M Lanham; D G Godfrey
Journal:  Exp Parasitol       Date:  1970-12       Impact factor: 2.011

4.  Seroprevalence of Trypanosoma evansi in dromedaries (Camelus dromedarius) from the Canary Islands (Spain) using an antibody Ab-ELISA.

Authors:  J M Molina; A Ruiz; M C Juste; J A Corbera; R Amador; C Gutiérrez
Journal:  Prev Vet Med       Date:  1999-10-19       Impact factor: 2.670

5.  Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam.

Authors:  D Verloo; W Holland; L N My; N G Thanh; P T Tam; B Goddeeris; J Vercruysse; P Büscher
Journal:  Vet Parasitol       Date:  2000-09-20       Impact factor: 2.738

6.  Development and application of an antibody-ELISA to follow up a Trypanosoma evansi outbreak in a dromedary camel herd in France.

Authors:  Marc Desquesnes; Géraldine Bossard; Sophie Thévenon; Delphine Patrel; Sophie Ravel; Djamila Pavlovic; Stéphane Herder; Olivier Patout; Elisabeth Lepetitcolin; Philippe Hollzmuller; David Berthier; Philippe Jacquiet; Gérard Cuny
Journal:  Vet Parasitol       Date:  2009-03-27       Impact factor: 2.738
  6 in total
  2 in total

1.  Haemato-biochemical and thyroxin status in Trypanosoma evansi infected dogs.

Authors:  B Sudhakara Reddy; K Nalini Kumari; S Sivajothi; V C Rayulu
Journal:  J Parasit Dis       Date:  2014-08-31

2.  Polypeptide profiles of South Indian isolate of Trypanosoma evansi.

Authors:  S Sivajothi; V C Rayulu; B V Bhaskar Reddy; P Malakondaiah; D Sreenivasulu; B Sudhakara Reddy
Journal:  J Parasit Dis       Date:  2014-09-05
  2 in total

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