BACKGROUND AND OBJECTIVE: To develop a safe and efficient surgical procedure for subretinal implantation into porcine eyes of a human embryonic stem cell-derived retinal pigmented epithelium (hESC-RPE) monolayer seeded onto a Parylene-C scaffold. This implant is referred to as CPCB-RPE1. MATERIALS AND METHODS: Ultrathin Parylene-C scaffolds were seeded with hESC-RPE and surgically implanted into the subretinal space of Yucatan mini pigs (n = 8). The surgery consisted of pars plana vitrectomy, induction of a limited retinal detachment, and peripheral retinotomy for insertion of the monolayer using a novel tissue injector, followed by silicone oil tamponade injection, laser photocoagulation around the retinotomy site, and inferior iridectomy. Oral cyclosporine was administered from day 1 and during the entire follow-up period. Three months later, the animals were euthanized and the eyes and major organs were submitted for histological analysis. Adjacent sections underwent immunohistochemical analysis to detect human cells using anti-TRA-1-85 (human blood group antigen) antibody and DAPI antibodies. RESULTS: The cell monolayer was immunopositive for TRA-1-85 3 months after implantation and migration from the Parylene-C scaffold was not detected. One eye had a mild inflammatory reaction around the implant that was negative for human biomarkers. No intraocular or systemic tumors were detected. CONCLUSION: The hESC-RPE cells survived for 3 months in this animal model. The surgical procedure for subretinal implantation of CPCB-RPE1 is feasible and safe, without cell migration off the scaffold or development of ocular or systemic tumors. Copyright 2016, SLACK Incorporated.
BACKGROUND AND OBJECTIVE: To develop a safe and efficient surgical procedure for subretinal implantation into porcine eyes of a human embryonic stem cell-derived retinal pigmented epithelium (hESC-RPE) monolayer seeded onto a Parylene-C scaffold. This implant is referred to as CPCB-RPE1. MATERIALS AND METHODS:Ultrathin Parylene-C scaffolds were seeded with hESC-RPE and surgically implanted into the subretinal space of Yucatan mini pigs (n = 8). The surgery consisted of pars plana vitrectomy, induction of a limited retinal detachment, and peripheral retinotomy for insertion of the monolayer using a novel tissue injector, followed by silicone oil tamponade injection, laser photocoagulation around the retinotomy site, and inferior iridectomy. Oral cyclosporine was administered from day 1 and during the entire follow-up period. Three months later, the animals were euthanized and the eyes and major organs were submitted for histological analysis. Adjacent sections underwent immunohistochemical analysis to detect human cells using anti-TRA-1-85 (human blood group antigen) antibody and DAPI antibodies. RESULTS: The cell monolayer was immunopositive for TRA-1-85 3 months after implantation and migration from the Parylene-C scaffold was not detected. One eye had a mild inflammatory reaction around the implant that was negative for human biomarkers. No intraocular or systemic tumors were detected. CONCLUSION: The hESC-RPE cells survived for 3 months in this animal model. The surgical procedure for subretinal implantation of CPCB-RPE1 is feasible and safe, without cell migration off the scaffold or development of ocular or systemic tumors. Copyright 2016, SLACK Incorporated.
Authors: Erin R Burnight; Joseph C Giacalone; Jessica A Cooke; Jessica R Thompson; Laura R Bohrer; Kathleen R Chirco; Arlene V Drack; John H Fingert; Kristan S Worthington; Luke A Wiley; Robert F Mullins; Edwin M Stone; Budd A Tucker Journal: Prog Retin Eye Res Date: 2018-03-22 Impact factor: 21.198
Authors: Wankun Xie; Min Zhao; Shu-Huai Tsai; William L Burkes; Luke B Potts; Wenjuan Xu; H Ross Payne; Travis W Hein; Lih Kuo; Robert H Rosa Journal: Exp Eye Res Date: 2018-08-16 Impact factor: 3.467
Authors: Fukutaro Mano; Jarel K Gandhi; Raphael Pereira da Silva; Aline Do Amaral Silva; Lucas Iezzi; Raymond Iezzi; Jose S Pulido; Alan D Marmorstein Journal: Transl Vis Sci Technol Date: 2022-04-01 Impact factor: 3.048
Authors: Amir H Kashani; Jane S Lebkowski; David R Hinton; Danhong Zhu; Mohamed A Faynus; Sanford Chen; Firas M Rahhal; Robert L Avery; Hani Salehi-Had; Clement Chan; Neal Palejwala; April Ingram; Wei Dang; Chih-Min Lin; Debbie Mitra; Juan Carlos Martinez-Camarillo; Jeff Bailey; Cassidy Arnold; Britney O Pennington; Narsing Rao; Lincoln V Johnson; Dennis O Clegg; Mark S Humayun Journal: Stem Cell Reports Date: 2022-02-03 Impact factor: 7.294
Authors: Fernando H Lojudice; Rodrigo A Brant Fernandes; Francesco Innocenti; Carlos E Franciozi; Priscila Cristovam; Maurício Maia; Mari C Sogayar; Rubens Belfort Junior Journal: Int J Retina Vitreous Date: 2019-10-21