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Literature DB >> 27062599

Measuring Redox Status of Melanoma Cells.

Feng Liu-Smith¹, Tatiana B Krasieva², Jing Liu³, Jiankang Liu⁴, Frank L Meyskens⁵.

Abstract

Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

Entities: Chemical Disease Gene Species

Keywords: Dichlorofluorescin diacetate (DCFDA); Dihydroethidium (DHE); Fluorometer; Hydrogen peroxide; Laser microscopy; Reactive oxygen species; Redox-sensitive GFP; Superoxide; ro1GFP; ro2GFP

Year: 2016 PMID： 27062599 PMCID： PMC5878701 DOI： 10.1007/7651_2016_352

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

12 in total

1. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators.

Authors: George T Hanson; Robert Aggeler; Devin Oglesbee; Mark Cannon; Roderick A Capaldi; Roger Y Tsien; S James Remington
Journal: J Biol Chem Date: 2004-01-13 Impact factor: 5.157

2. Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators.

Authors: Colette T Dooley; Timothy M Dore; George T Hanson; W Coyt Jackson; S James Remington; Roger Y Tsien
Journal: J Biol Chem Date: 2004-02-25 Impact factor: 5.157

Review 3. Redox-sensitive green fluorescent protein: probes for dynamic intracellular redox responses. A review.

Authors: Mark B Cannon; S James Remington
Journal: Methods Mol Biol Date: 2008

Review 4. Fluorescent protein-based redox probes.

Authors: Andreas J Meyer; Tobias P Dick
Journal: Antioxid Redox Signal Date: 2010-09-01 Impact factor: 8.401

Review 5. Reactive oxygen species (ROS)--induced genetic and epigenetic alterations in human carcinogenesis.

Authors: Dominique Ziech; Rodrigo Franco; Aglaia Pappa; Mihalis I Panayiotidis
Journal: Mutat Res Date: 2011-03-16 Impact factor: 2.433

6. Evidence for free radical formation during the oxidation of 2'-7'-dichlorofluorescin to the fluorescent dye 2'-7'-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements.

Authors: C Rota; C F Chignell; R P Mason
Journal: Free Radic Biol Med Date: 1999-10 Impact factor: 7.376

Review 7. Involvement of thio-, peroxi-, and glutaredoxins in cellular redox-dependent processes.

Authors: E V Kalinina; N N Chernov; A N Saprin
Journal: Biochemistry (Mosc) Date: 2008-12 Impact factor: 2.487

8. Analysis of dihydroethidium fluorescence for the detection of intracellular and extracellular superoxide produced by NADPH oxidase.

Authors: Hitesh M Peshavariya; Gregory James Dusting; Stavros Selemidis
Journal: Free Radic Res Date: 2007-06

Measuring Redox Status of Melanoma Cells.

1. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators.

2. Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators.

Review 3. Redox-sensitive green fluorescent protein: probes for dynamic intracellular redox responses. A review.

Review 4. Fluorescent protein-based redox probes.

Review 5. Reactive oxygen species (ROS)--induced genetic and epigenetic alterations in human carcinogenesis.

6. Evidence for free radical formation during the oxidation of 2'-7'-dichlorofluorescin to the fluorescent dye 2'-7'-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements.

Review 7. Involvement of thio-, peroxi-, and glutaredoxins in cellular redox-dependent processes.

8. Analysis of dihydroethidium fluorescence for the detection of intracellular and extracellular superoxide produced by NADPH oxidase.

9. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.

10. Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise.

Review 1. Autophagy: In the cROSshairs of cancer.

2. Effects of Aluminum on the Integrity of the Intestinal Epithelium: An in Vitro and in Vivo Study.