James E Bupp1, Mary J Tanga1. 1. Biosciences Division, SRI International, 333 Ravenswood Ave, Menlo Park, CA, 94025, USA.
Abstract
A stable isotope labelled mass spectrometry internal standard of the antibiotic doxycycline was prepared to assist in pharmacokinetic analyses. Our approach was to first N-demethylate doxycycline using a non-classical Polonovski reaction and then re-methylate using methyl-[(13) CD3 ] iodide, which gave doxycycline-[(13) CD3 ] with an isotopic purity of 99%.
A stable isotope labelled mass spectrometry internal standard of the antibiotic doxycycline was prepared to assist in pharmacokinetic analyses. Our approach was to first n class="Chemical">N-demethylate doxycycline using a non-classical Polonovski reaction and then re-methylate using methyl-[(13) CD3 ] iodide, which gave doxycycline-[(13) CD3 ] with an isotopic purity of 99%.
Authors: David R Hill; Charles D Ericsson; Richard D Pearson; Jay S Keystone; David O Freedman; Phyllis E Kozarsky; Herbert L DuPont; Frank J Bia; Philip R Fischer; Edward T Ryan Journal: Clin Infect Dis Date: 2006-11-08 Impact factor: 9.079