Literature DB >> 27060444

Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth.

Kássia de Carvalho Dias1, Paula Aboud Barbugli2, Carlos Eduardo Vergani3.   

Abstract

This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.
Copyright © 2016. Published by Elsevier B.V.

Entities:  

Keywords:  Candida albicans; Cell survival; HEPES; Keratinocytes; Staphylococcus aureus

Mesh:

Substances:

Year:  2016        PMID: 27060444     DOI: 10.1016/j.mimet.2016.03.018

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  7 in total

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