Mayna Adabbo1, Fernanda Rossi Paolillo2, Paulo Sérgio Bossini3, Natalia Camargo Rodrigues4, Vanderlei Salvador Bagnato2, Nivaldo Antonio Parizotto3. 1. 1 Biotechnology Program, Federal University of São Carlos (UFSCar) , São Carlos, SP, Brazil . 2. 2 Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP) , São Carlos, SP, Brazil . 3. 3 Electrothermophototherapy Laboratory, Department of Physical Therapy, Federal University of São Carlos (UFSCar) , São Carlos, SP, Brazil . 4. 4 Department of Biomechanics, Medicine and Rehabilitation of Locomotor System, University of São Paulo (USP) , School of Medicine, Ribeirão Preto, SP, Brazil .
Abstract
OBJECTIVE: The aim of this study was to analyze the effects of low-level laser therapy (LLLT) when associated with treadmill training on the recovery of skeletal muscle, during two periods of rest after muscle injury in rats. BACKGROUND DATA: Because of photostimulation, LLLT has been presented as an alternative for accelerating the tissue healing process. MATERIALS AND METHODS: Forty rats were divided into two groups (A and B) containing four subgroups each: GC (Control Group)-cryolesion untreated; EG (Exercise Group)-cryolesion treated with physical exercise; LG (Laser Group)-cryolesion treated with laser; ELG (Exercise and Laser Group)-cryolesion treated with laser and physical exercise. The right tibialis anterior (TA) of the middle belly was injured by a cooling iron bar (cryoinjury). Group A remained at rest for 3 days, whereas Group B remained at rest for 7 days. The laser parameters utilized were 780 nm with 15 mW average optical power and spot size of 0.04 cm(2) applied during 10 sec, leading to 0.152 J and 3.8 J/cm(2). Treadmill training with and without laser application was performed during 5 days, with each session lasting for 12 min at a velocity of 17 m/min. Subsequently, the TA muscle was removed for a histological and morphometric analysis. RESULTS: The damaged area was significantly smaller for the ELG at both periods of rest, 3 and 7 days, respectively (4.4 ± 0.42% and 3.5 ± 0.14%, p < 0.05), when compared with the LG (18.6 ± 0.64% and 7.5 ± 0.13%), the EG (21 ± 0.26% and 8.7 ± 0.32%), and the CG (23.9 ± 0.37% and 21.4 ± 0.38%). In addition, the number of blood vessels were significantly higher for the ELG at both periods of rest, 3 and 7 days, respectively (71.2 ± 13.51 and 104.5 ± 11.78, p < 0.05), when compared with the LG (60.6 ± 11.25 and 93.5 ± 16.87), the EG (51.6 ± 7.3 and 93.8 ± 15.1) and the CG (34.4 ± 2.54 and 65.7 ± 14.1). CONCLUSIONS: The LLLT applied before the physical exercise on the treadmill stimulated the angiogenesis and accelerated the process of muscle recovery.
OBJECTIVE: The aim of this study was to analyze the effects of low-level laser therapy (LLLT) when associated with treadmill training on the recovery of skeletal muscle, during two periods of rest after muscle injury in rats. BACKGROUND DATA: Because of photostimulation, LLLT has been presented as an alternative for accelerating the tissue healing process. MATERIALS AND METHODS: Forty rats were divided into two groups (A and B) containing four subgroups each: GC (Control Group)-cryolesion untreated; EG (Exercise Group)-cryolesion treated with physical exercise; LG (Laser Group)-cryolesion treated with laser; ELG (Exercise and Laser Group)-cryolesion treated with laser and physical exercise. The right tibialis anterior (TA) of the middle belly was injured by a cooling iron bar (cryoinjury). Group A remained at rest for 3 days, whereas Group B remained at rest for 7 days. The laser parameters utilized were 780 nm with 15 mW average optical power and spot size of 0.04 cm(2) applied during 10 sec, leading to 0.152 J and 3.8 J/cm(2). Treadmill training with and without laser application was performed during 5 days, with each session lasting for 12 min at a velocity of 17 m/min. Subsequently, the TA muscle was removed for a histological and morphometric analysis. RESULTS: The damaged area was significantly smaller for the ELG at both periods of rest, 3 and 7 days, respectively (4.4 ± 0.42% and 3.5 ± 0.14%, p < 0.05), when compared with the LG (18.6 ± 0.64% and 7.5 ± 0.13%), the EG (21 ± 0.26% and 8.7 ± 0.32%), and the CG (23.9 ± 0.37% and 21.4 ± 0.38%). In addition, the number of blood vessels were significantly higher for the ELG at both periods of rest, 3 and 7 days, respectively (71.2 ± 13.51 and 104.5 ± 11.78, p < 0.05), when compared with the LG (60.6 ± 11.25 and 93.5 ± 16.87), the EG (51.6 ± 7.3 and 93.8 ± 15.1) and the CG (34.4 ± 2.54 and 65.7 ± 14.1). CONCLUSIONS: The LLLT applied before the physical exercise on the treadmill stimulated the angiogenesis and accelerated the process of muscle recovery.
Authors: F R Paolillo; A V Corazza; A R Paolillo; A Borghi-Silva; R Arena; C Kurachi; V S Bagnato Journal: Climacteric Date: 2013-09-03 Impact factor: 3.005
Authors: Roberta M Brunelli; Natalia C Rodrigues; Daniel A Ribeiro; Kelly Fernandes; Angela Magri; Lívia Assis; Nivaldo A Parizotto; Alberto Cliquet; Ana Claudia M Renno; Daniela C C Abreu Journal: Lasers Med Sci Date: 2013-02-14 Impact factor: 3.161
Authors: Lívia Assis; Ana Iochabel Soares Moretti; Thalita Balsamo Abrahão; Heraldo Possolo de Souza; Michael R Hamblin; Nivaldo Antonio Parizotto Journal: Lasers Med Sci Date: 2012-08-17 Impact factor: 3.161
Authors: Kristen A Baltgalvis; Jarrod A Call; Gregory D Cochrane; Rhianna C Laker; Zhen Yan; Dawn A Lowe Journal: Med Sci Sports Exerc Date: 2012-09 Impact factor: 5.411
Authors: Tero A H Järvinen; Teppo L N Järvinen; Minna Kääriäinen; Ville Aärimaa; Samuli Vaittinen; Hannu Kalimo; Markku Järvinen Journal: Best Pract Res Clin Rheumatol Date: 2007-04 Impact factor: 4.098
Authors: Vanessa Batista da Costa Santos; Solange de Paula Ramos; Vinícius Flávio Milanez; Julio Cesar Molina Corrêa; Rubens Igor de Andrade Alves; Ivan Frederico Lupiano Dias; Fábio Yuzo Nakamura Journal: Lasers Med Sci Date: 2013-06-19 Impact factor: 3.161
Authors: Trisha D Scribbans; Brittany A Edgett; Kira Vorobej; Andrew S Mitchell; Sophie D Joanisse; Jennifer B L Matusiak; Gianni Parise; Joe Quadrilatero; Brendon J Gurd Journal: PLoS One Date: 2014-06-05 Impact factor: 3.240
Authors: Gavriella Shefer; Terry A Partridge; Louise Heslop; Jacqueline G Gross; Uri Oron; Orna Halevy Journal: J Cell Sci Date: 2002-04-01 Impact factor: 5.285